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  4. Recombinant Human DNA topoisomerase 3-beta-1 (TOP3B), partial

Recombinant Human DNA topoisomerase 3-beta-1 (TOP3B), partial

  • 中文名称：
    人TOP3B重组蛋白
  • 货号：
    CSB-YP024063HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    人TOP3B重组蛋白
  • 货号：
    CSB-EP024063HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    人TOP3B重组蛋白
  • 货号：
    CSB-EP024063HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    人TOP3B重组蛋白
  • 货号：
    CSB-BP024063HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    人TOP3B重组蛋白
  • 货号：
    CSB-MP024063HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    TOP3B
  • Uniprot No.：
    O95985
  • 别名：
    DNA topoisomerase 3 beta 1; DNA topoisomerase 3-beta-1; DNA topoisomerase III beta 1; DNA topoisomerase III beta-1; FLJ39376; TOP 3B; TOP3B; TOP3B_HUMAN; TOP3B1; Topo III beta; Topoisomerase (DNA) III beta; Topoisomerase DNA III beta; Topoisomerase III beta
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

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TOP3B antibodies

靶点详情

  • 功能：
    Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Possesses negatively supercoiled DNA relaxing activity.
  • 基因功能参考文献：
    1. Structural basis of the interaction between TOP3B and the TDRD3 auxiliary factor has been reported. PMID: 28176834
    2. Results identified TOP3B gene deletion as candidate gene in the etiology of neurodevelopmental disorders including juvenile myoclonic epilepsy. PMID: 29490292
    3. Top3beta mutants lacking either its RNA-binding domain or catalytic residue fail to promote synapse formation, suggesting that Top3beta requires both its mRNA-binding and catalytic activity to facilitate neurodevelopment. PMID: 28039324
    4. Top3b proteins from several animals associate with polyribosomes, which are units of mRNA translation, whereas the Top3 homologs from E. coli and yeast lack the association. The Top3b-polyribosome association requires TDRD3, which directly interacts with Top3beta and is present in animals but not bacteria or yeast. PMID: 27257063
    5. TOP3B promotes transcription, protects against DNA damage, and reduces the frequency of chromosomal translocations. PMID: 24507716
    6. Topoisomerase IIIbeta expression can be a useful marker in assessing the prognosis of patients with breast cancer and is an independent predictor of survival. PMID: 20950730
    7. the positive regulatory region including the 5'GGAACC3' core element is essential for efficient expression of the DNA topoisomerase III beta gene PMID: 15358519
    8. Two isoforms of human DNA topoisomerase IIIbeta were expressed in HeLa cells. Isoform 1 was localized to the nucleus and associated with chromosomes during metaphase and anaphase. Isoform 2 was found in the cytoplasm. PMID: 11549288

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  • 蛋白家族：
    Type IA topoisomerase family
  • 组织特异性：
    Isoform 1 is found in testis, heart and skeletal muscle. A 4 kb transcript which probably represents isoform 2 is found in thymus, kidney and pancreas.
  • 数据库链接：

    HGNC: 11993

    OMIM: 603582

    KEGG: hsa:8940

    STRING: 9606.ENSP00000349705

    UniGene: Hs.436401

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