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  4. Recombinant Human Cytoplasmic aconitate hydratase (ACO1), partial

Recombinant Human Cytoplasmic aconitate hydratase (ACO1), partial

  • 中文名称：
    Recombinant Human Cytoplasmic aconitate hydratase(ACO1) ,partial
  • 货号：
    CSB-YP001161HU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Human Cytoplasmic aconitate hydratase(ACO1) ,partial
  • 货号：
    CSB-EP001161HU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Human Cytoplasmic aconitate hydratase(ACO1) ,partial
  • 货号：
    CSB-EP001161HU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Human Cytoplasmic aconitate hydratase(ACO1) ,partial
  • 货号：
    CSB-BP001161HU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Human Cytoplasmic aconitate hydratase(ACO1) ,partial
  • 货号：
    CSB-MP001161HU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    ACO1
  • Uniprot No.：
    P21399
  • 别名：
    ACO 1; ACO1; ACOC_HUMAN; Aconitase 1 soluble; Aconitase; Aconitase1; Aconitate hydratase; ACONS; Citrate hydro lyase; Citrate hydro-lyase; Cytoplasmic aconitate hydratase; Ferritin repressor protein; IRE BP 1; IRE-BP 1; IREB 1; IREB1; IREBP; IREBP1; Iron regulatory protein 1; Iron responsive element binding protein 1; Iron-responsive element-binding protein 1; IRP 1; IRP1; OTTHUMP00000045233
  • 种属：
    Homo sapiens (Human)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

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ACO1 antibodies

问答及客户评论

■ 常见问题解答
Q:

Please can you provide information for all available sizes?
I would like to know if you have received any customer feedback that the protein has binding activity,

A:
Thanks for your inquiry.
Expression Region: 693-818aa; Partial.
Tag information:Tag type will be determined during the manufacturing process.
The expected tag is N-terminal 10xHis-tagged and C-terminal Myc-tagged.
Sequence:

ARYLTNRGLTPREFNSYGSRRGNDAVMARGTFANIRLLNRFLNKQAPQTIHLPSGEILDVFDAAERYQQAGLPLIVLAGKEYGAGSSRDWAAKGPFLLGIKAVLAESYERIHRSNLVGMGVIPLEY


Sorry, we haven't received any feedback about the binding activity of this protein up to now, and we found there is very a few reference about its activity.Sorry, we can't provide any useful information, but we recommend you to purchase a small size of eukaryotic expressed protein to have a try,it should be more likely to be active.
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靶点详情

  • 功能：
    Bifunctional iron sensor that switches between 2 activities depending on iron availability. Iron deprivation, promotes its mRNA binding activity through which it regulates the expression of genes involved in iron uptake, sequestration and utilization. Binds to iron-responsive elements (IRES) in the untranslated region of target mRNAs preventing for instance the translation of ferritin and aminolevulinic acid synthase and stabilizing the transferrin receptor mRNA.; Conversely, when cellular iron levels are high, binds a 4Fe-4S cluster which precludes RNA binding activity and promotes the aconitase activity, the isomerization of citrate to isocitrate via cis-aconitate.
  • 基因功能参考文献：
    1. These results support the concept that IRP1 is an oxidative stress biosensor that mediates iron accumulation and cell death when deregulated by mitochondrial dysfunction. PMID: 28502703
    2. Results indicate that there is a dysregulation of DMT1 + IRE in IA testes, which might due to the up-regulation of IRP1 and HIF-1A. PMID: 28762519
    3. Evaluation of the iron regulatory protein-1 interactome has been presented. PMID: 29330752
    4. regulatory circuit involving FBXL5 and CIA acts through both IRPs to control iron metabolism and promote optimal cell growth PMID: 28768766
    5. Data suggest that Fe-S cluster of IRP1 plays role in sensing/regulating cellular iron homeostasis; IRP1 alternates between function as cytosolic aconitase [which contains Fe(4)-S(4) cluster in active-site cleft] and function as apoenzyme [which lacks Fe-S cluster] that binds to iron response element stem-loop structures present in several iron regulatory protein transcripts. [REVIEW] PMID: 28615439
    6. The SNP rs7874815 in the ACO1 gene was strongly associated with survival in pancreatic cancer. PMID: 26755275
    7. Our results confirm the observation that mitoNEET is important in transferring the iron sulfur clusters to the cytosolic aconitase in living cells and the His-87 ligand in mitoNEET plays important role in this process. PMID: 26778000
    8. Data show that IRP1 suppressed TfR1/DMT1 (+IRE) expression, limited the cellular iron content and decreased lactate dehydrogenase (LDH) release induced by hypoxia. PMID: 25727755
    9. These in vitro studies suggest that human GLRX3 is important for cytosolic Fe-S protein maturation. PMID: 26296460
    10. SIRT3 overexpression decreases TfR1 expression by inhibiting IRP1 and represses proliferation in pancreatic cancer cells. PMID: 24909164
    11. ACO1 gene expression was positively associated with adipogenic markers in subcutaneous and visceral adipose tissue. PMID: 25550467
    12. ACO1 genetic variation is associated with neuropathic pain and pain severity in HIV-infected patients on antiretroviral therapy PMID: 25144566
    13. MitoNEET governs a novel trafficking pathway to rebuild an Fe-S cluster into cytosolic aconitase/IRP1. PMID: 25012650
    14. Polymer-dependent translocation of IRP-1 in Cd(2+)-exposed cells may underlie effects of Cd(2+) on iron homeostasis. PMID: 25106854
    15. Data indicate that JTR-009, a benzimidazole, operated by preventing iron-regulatory protein-1 (IRP1) from binding to the iron-responsive element (IRE) in APP mRNA. PMID: 23935819
    16. Aco1 encodes a moonlighting protein that has aconitase activity as well as mRNA binding ability. PMID: 8041788
    17. Data indicate that silencing of Grx3 in HeLa cells decreases the activities of several cytosolic Fe/S proteins, including iron-regulatory protein 1, a major component of posttranscriptional iron regulation. PMID: 23615448
    18. Studies indicate that several genes have been linked to iron homeostasis, including transferrin (TF), iron regulatory protein 1 (ACO1) and transferrin receptor 2 (TFR2). PMID: 23817740
    19. Lead specifically induces dysregulation of IRP1 protein by activating the ERK1/2 signaling pathway indicating a novel role for IRP1 and the ERK/MAPK pathway in vascular endothelial functions. PMID: 22502979
    20. The RNA-binding and aconitase forms of IRP-1 can undergo interconversion dependent on the density of cells growing in culture. PMID: 22544036
    21. Ggenetic polymorphisms of the iron regulatory protein 1 and 2 genes may be associated with development of age-related macular degeneration. PMID: 22331484
    22. Molecular genetic analysis revealed point mutations within the FTL IRE. PMID: 22020773
    23. Dual-luciferase reporter assay showed that iron regulatory protein 1 (IRP)1 was directly downregulated by hypoxia-inducible factor 1/hypoxia response element system. PMID: 21360641
    24. Single nucleotide polymorphism in ACO1 gene is associated with cutaneous malignant melanoma. PMID: 20574843
    25. Selective translational control of the Alzheimer amyloid precursor protein transcript by iron regulatory protein-1. PMID: 20558735
    26. ATP/ADP binding maintains IRP-1 in a non-IRE-binding state. PMID: 20569198
    27. iron regulatory protein-1-mediated inhibition of hypoxia-inducible factor-2a translation is linked to the anti-inflammatory 15-deoxy-delta12,14-prostaglandin J2 PMID: 20354189
    28. gene coding and flanking regions were sequenced and examined for mutations that might modulate the iron burden of individuals harboring the common mutant hemochromatosis HFE genotype or cause hemochromatosis independent of mutations in the HFE gene PMID: 11783942
    29. Structural changes associated with switching activities of human iron regulatory protein 1 PMID: 11812787
    30. liver levels or this protein are down-regulated in hemochromatosis PMID: 12196178
    31. functional in the activation of iron uptake PMID: 12572667
    32. data suggest that the differential regulation of IRP1 and IRP2 during hypoxia may be important for cellular adaptation to low oxygen tension. PMID: 12855587
    33. A phosphomimetic mutation at Ser-138 renders IRP1 sensitive to iron-dependent degradation. PMID: 12972614
    34. Toxoplasma gondii infection resulted in increased activity in the iron response protein IRP1, which, in this state, stabilizes transferrin receptor mRNA from degradation. PMID: 15349772
    35. iron-replete HepG2 cells exhibit a narrow range of maximal responsiveness of the IRP-regulatory mechanism PMID: 15543932
    36. Apo-IRP1 exists as an equilibrium mixture of monomers and dimers in solution; however, only monomeric IRP1 is observed in complex with the iron regulatory element (IRE). PMID: 15938636
    37. Comparison of IRP1 in its aconitase form with known structures of homologous enzymes reveals well-conserved folds and active site environments with significantly different surface shapes and charge distributions. PMID: 16407072
    38. IRP1 controls iron homeostasis during cell proliferation, inflammation, and in response to diseases altering cytosolic Fe-S cluster assembly or disassembly. PMID: 16424901
    39. IRP1 activity level is higher in monocytes and macrophages of hereditary hemochrosis (HH) patients than in those of control subjects, and is increased in the duodenal samples of the patients with HH and iron-deficiency anemia. PMID: 16503547
    40. Overexpression of iron regulatory protein 1 suppresses growth of tumor xenografts PMID: 17127713
    41. Results describe thet effect of hypoxia on the binding and subcellular distribution of iron regulatory proteins 1 and 2. PMID: 17200797
    42. Thus, proper folding of human iron regulatory protein 1 strongly depends on its cellular environment, in contrast to other members of the aconitase family. PMID: 17244191
    43. Although the iron-responsive element does not itself hydrolyze ATP, its presence enhances iron regulatory protein-1's ATPase activity, and ATP hydrolysis results in loss of the complex in gel shift assays. PMID: 17521334
    44. analysis of the iron regulatory protein system PMID: 17604281
    45. Hypertransfusional (>8 transfusions/year) iron in liver biopsies correlated with increased expression (RNA) for iron regulatory proteins 1 and 2 (3-, 9- to 11-fold) and hepcidin RNA: (5- to 8-fold), while ferritin H and L RNA remained constant. PMID: 17613866
    46. Expression of TfR1 mRNA is increased when maternal iron deficiency progressed while there is no change in the expression of IRP1 mRNA in the placenta. PMID: 17877204
    47. Downregulated by HBX in human hepatoma cell lines. PMID: 18262302
    48. This study shows that expression of the transferrin receptor (TfR) is downregulated, whereas expression of the ferritin heavy chain is upregulated in Chlamydia trachomatis infected HeLa-229 cells. PMID: 19688112

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  • 亚细胞定位：
    Cytoplasm, cytosol.
  • 蛋白家族：
    Aconitase/IPM isomerase family
  • 数据库链接：

    HGNC: 117

    OMIM: 100880

    KEGG: hsa:48

    STRING: 9606.ENSP00000309477

    UniGene: Hs.567229

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