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  4. Recombinant Halobacterium salinarum Aspartate--tRNA ligase (aspS)

Recombinant Halobacterium salinarum Aspartate--tRNA ligase (aspS)

  • 中文名称：
    Recombinant Halobacterium salinarum Aspartate--tRNA ligase(aspS)
  • 货号：
    CSB-YP515483HTM
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Halobacterium salinarum Aspartate--tRNA ligase(aspS)
  • 货号：
    CSB-EP515483HTM
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Halobacterium salinarum Aspartate--tRNA ligase(aspS)
  • 货号：
    CSB-EP515483HTM-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Halobacterium salinarum Aspartate--tRNA ligase(aspS)
  • 货号：
    CSB-BP515483HTM
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Halobacterium salinarum Aspartate--tRNA ligase(aspS)
  • 货号：
    CSB-MP515483HTM
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    aspS
  • Uniprot No.：
    O07683
  • 别名：
    aspS; VNG_0461G; Aspartate--tRNA(Asp/Asn) ligase; EC 6.1.1.23; Aspartyl-tRNA synthetase; AspRS; Non-discriminating aspartyl-tRNA synthetase; ND-AspRS
  • 种属：
    Halobacterium salinarum (strain ATCC 700922 / JCM 11081 / NRC-1) (Halobacterium halobium)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-436
  • 氨基酸序列
    MLERTYIEDV TPDDDGEDTT IAGHVHELRD LGGILFVIVR DATGRLQVVA KEDDTPDLFE TAEGLSSEDV VQVTGTLEAS DQAPGGVELA PTELTVVSEA TDVPSIEISK DVDADLSTRL DERHLDVRKP STKAVFSLRS KAMGAMTDWF YDNRFEEVDT PELSTAGAEG GADLFPVVYY DKEAYLSQSP QLYKQILVAS GVDRLFEVGH AFRAEDFGTS RHVSEIAMFD VELGYVEDHH DVMDVQEESL RHTIRHVVEH AQRELDELGS DLSVPEADFP RITFEEAREI LADEYDHVPE DDNDLDTKGE RLLGEYFEEQ GHPAVFVVGY PDEKFYYRQD VDGDDVASRK FDLLYRGQEL SSGGQREHDI ERLTAKMREQ GVEPENFEFY LDAFRYGVPP HGGYGLGIDR LIQQLAGLDN IKEAILFPRD PDRLEP
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Aspartyl-tRNA synthetase with relaxed tRNA specificity since it is able to aspartylate not only its cognate tRNA(Asp) but also tRNA(Asn). This indicates that it is not able to differentiate between base C36 in the tRNA(Asp) anticodon (GUC) from base U36 in the tRNA(Asn) (anticodon GUU). Reaction proceeds in two steps: L-aspartate is first activated by ATP to form Asp-AMP and then transferred to the acceptor end of tRNA(Asp/Asn).
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Class-II aminoacyl-tRNA synthetase family, Type 2 subfamily
  • 数据库链接：

    KEGG: hal:VNG_0461G

    STRING: 64091.VNG0461G

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