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  4. Recombinant Forkhead box protein O (daf-16), partial

Recombinant Forkhead box protein O (daf-16), partial

  • 中文名称：
    Recombinant Forkhead box protein O(daf-16) ,partial
  • 货号：
    CSB-YP519425CXY
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Forkhead box protein O(daf-16) ,partial
  • 货号：
    CSB-EP519425CXY
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Forkhead box protein O(daf-16) ,partial
  • 货号：
    CSB-EP519425CXY-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Forkhead box protein O(daf-16) ,partial
  • 货号：
    CSB-BP519425CXY
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Forkhead box protein O(daf-16) ,partial
  • 货号：
    CSB-MP519425CXY
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    daf-16
  • Uniprot No.：
    O16850
  • 别名：
    daf-16; R13H8.1Forkhead box protein O; FOXO; Abnormal dauer formation protein 16
  • 种属：
    Caenorhabditis elegans
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Forkhead-type transcription factor. Binds to the promoters of genes that contain the daf-16 binding element (DBE), TTGTTTAC, in their regulatory region. Functions in the Insulin/IGF-1-like signaling (IIS) mediated pathway which affects lipogenesis, lifespan, starvation survival, heat shock and oxidative stress responses, sleep, and dauer formation. Longevity signaling predominantly arises from expression in the intestine. Daf-16 transcriptional activity is negatively regulated by cytoplasmic sequestration by association with ftt-2. Inhibition is required for the carbon dioxide (CO2) avoidance response. Upon loss of inhibition, daf-16 translocates to the nucleus to regulate genes that result in delayed reproduction and growth while increasing stress resistance starvation tolerance and longevity. Association with arginine methyltransferase prmt-1 prevents phosphorylation and allows for translocation to the nucleus and the subsequent transcription of longevity-related genes. Modulation of its activity by cGMP levels in sensory neurons regulates lifespan. Has a protective role against muscle dystrophy. Involved in mediating protection against aberrant protein aggregation proteotoxicity. Influences transcription of genes that code for proteins involved in immunity as part of a general stress response. Targets genes that inhibit and stimulate tumor growth. Targets kinases, phosphatases and transcription factors that are primarily involved in signaling and gene regulation. Thought to regulate ins-7 in FOXO-to-FOXO signaling, which coordinates daf-16 expression. Activity is positively regulated by shc-1-mediated inhibition of daf-2 and activation of JNK pathway. Functions by indirect interaction with jnk-1 of the mitogen-activated protein kinase (MAPK) pathway. Involved in increased proteasome activity by activating expression of rpn-6.1 in response to proteotoxic stress, leading to enhanced assembly of the 26S proteasome, followed by higher proteasome activity. Also regulates proteasome activity in the intestine by preventing expression of deubiquitinase ubh-4. Represses transcription of natc-1. Involved in regulation of srh-234 expression. Binds to the promoter of the AMPK-gamma regulatory subunit, aakg-4, and activates its transcription. Also activates transcription of AMPK-gamma regulatory subunit, aakg-1. Maintains endoplasmic reticulum (ER) function by inducing protein degradation and elimination to remove misfolded secretory proteins from the ER independently of the ire-1/xbp-1 unfolded protein response pathway. Regulates epidermal innate immunity to nematophagous fungal infection and physical wounding which trigger bli-3 induced ROS release, leading to daf-16 activation independently of daf-2 signaling. May negatively regulate resistance to stress caused by oxidized cholesterol adducts by preventing the activation of daf-9 and nuclear hormone receptor daf-12, two members of the steroid signaling pathway. Promotes apoptosis during embryonic development. Probably through the regulation of the autophagy genes atg-18 and atg-16.2, plays a role in regulating stem cell number in the germline during larval development. Plays a role in a type of aversive gustatory associated learning called salt avoidance learning. Plays a role in regulating gene transcription in response to white light exposure. Binds to the promoter of dex-1 to positively regulate its expression in seam cells during the dauer phase.
  • 基因功能参考文献：
    1. these data highlights the dependence on a daf-16/FOXO-interaction network as a common feature of several compound families for prolonging neuronal function in Huntington's disease. PMID: 28638078
    2. These data indicate that activation of DAF-16/FOXO3A is a highly conserved response to genotoxic stress that is important for suppressing consequent oxidative stress. Correspondingly, dysregulation of DAF-16/FOXO3A appears to underpin shortened healthspan and lifespan, rather than the increased DNA damage burden itself. PMID: 30031267
    3. our results indicate that Methyl 3,4-dihydroxybenzoate (MDHB) at least partially acts as a modulator of the daf-2/daf-16 pathway to extend the lifespan of C. elegans, and MDHB might be a promising therapeutic agent for age-related diseases PMID: 29874838
    4. mir-259 functioned in pharynx-intestinal valve and RSKS-1 functioned in pharynx to regulate MWCNTs toxicity. Furthermore, RSKS-1 regulated MWCNTs toxicity by suppressing the function of AAK-2-DAF-16 signaling cascade. Our results will strengthen our understanding the microRNAs mediated protection mechanisms for animals against the toxicity from certain nanomaterials. PMID: 27573184
    5. skn-1 is required for Oxr but not increased lifespan resulting from over-expression of DAF-16; concomitantly, DAF-16 over-expression rescues the short lifespan of skn-1 mutants but not their hypersensitivity to oxidative stress. PMID: 28612944
    6. mir-34 provides robustness to stress response programs by controlling noise in the DAF-16/FOXO-regulated gene network. PMID: 27905558
    7. This study indicated that lifespan extension and enhanced stress resistance induced by piceatannol requires DAF-16. PMID: 28128482
    8. involved in the immune response elicited by the host during the Shigella flexneri infection PMID: 27838822
    9. In the intestine of reproducing worms, DAF-16 promotes acidic lysosomes by upregulating the expression of v-ATPase genes. PMID: 28696216
    10. Results suggest that DAF-16 direct targets might play global roles in lifespan regulation. PMID: 27027346
    11. results of this study demonstrate a central role of PMK-1 for the processing of cellular responses to abiotic and biotic stressors, with the promoting effects of PMK-1 on Cd resistance mostly mediated by the transcription factors SKN-1 and DAF-16 PMID: 28766017
    12. turnover of individual proteins in the long-lived daf-2 mutant, is reported. PMID: 27626670
    13. Genetic analyses showed recombinant buckwheat trypsin inhibitor (rBTI) increased the transcriptional activity of DAF-16 and the disruption of daf-16 abolished rBTI-mediated protective effect in AD worms. rBTI promoted the autophagy-lysosomal degradation pathway to reduce the Abeta-induced toxicity via DAF-16 in an Alzheimer's disease model C. elegans, implying that BTI has the potential to protect against AD. PMID: 28119052
    14. Cholesterol plays an important role in the fasting-induced nuclear accumulation of DAF-16. Moreover, knockdown of the cholesterol-binding protein NSBP-1, which has been shown to bind to DAF-16 in a cholesterol-dependent manner and to regulate DAF-16 activity, suppresses both fasting-induced longevity and DAF-16 nuclear accumulation. PMID: 27989925
    15. Pyrroloquinoline quinone (PQQ) enhances resistance to oxidative stress and extends the lifespan of C. elegans. The mechanism concerning the lifespan extension may be dependent on increased activities of DAF-16/FOXO and SKN-1/Nrf2. PMID: 27090484
    16. This study found evidences that the transcription factor DAF-16/FOXO was involved in both oleic acid- and C. tenuifolia seed oil-mediated oxidative stress resistance in C. elegans. PMID: 27275864
    17. Direct binding of DAF-16 and R-SMAD proteins helps to activate genes involved in the mTORC1 signaling pathway that is frequently activated in tumors. PMID: 28549065
    18. Data show that isoamyl alcohol stimulus promoted longevity in a forkhead box O protein DAF-16-dependent manner, on the other hand, acetic acid stimulus promoted thermotolerance through mechanisms independent of DAF-16. PMID: 28209513
    19. the LIN-28/let-7/AKT/DAF-16 axis is a program that plays an important role in balancing reproduction and somatic maintenance. PMID: 27730721
    20. We consider irg-7 to be an integral downstream component of the germline longevity pathway because its expression increases upon germ cell removal and its depletion interferes with the activation of the longevity-promoting transcription factors DAF-16 and DAF-12 in germlineless animals. PMID: 28196094
    21. We demonstrate that while DAF-16/FOXO is dispensable, the age-dependent suppression of cilia phenotypes in IFT mutants requires cell-autonomous functions of the HSF1 heat shock factor and the Hsp90 chaperone PMID: 27906968
    22. we found that Octopamine administration promoted the nuclear translocation of DAF-16, the key transcription factor in fasting responses, and that the Octopamine -induced enhancement of stress resistance required DAF-16. PMID: 28105749
    23. Thus, M084 might inhibit the mitochondrial respiration, activate mitochondrial unfolded protein response and AMPK, recruite SIR-2.1 and SKN-1, and finally through the transcription factor DAF-16, delay the aging process of C. elegans. PMID: 27854075
    24. baicalein increases stress-resistance and life span in C. elegans via SKN-1 but not DAF-16 PMID: 27370100
    25. Report transcriptional biology of FOXO/DAF-16 and gene regulation downstream of the insulin signaling pathway. PMID: 26539642
    26. The function of these FOXO proteins in age-related diseases has been extensively studied, in the model organism Caenorhabditis elegans as well as in humans. PMID: 26363351
    27. the C. elegans 44 bp enhancer from vit-2 vitellogenin gene is directly regulated by ELT-2, MAB-3, FKH-9 and DAF-16 and indirectly regulated by the germline, by daf-2/insulin signaling and by the TGF-beta/Sma/Mab pathway PMID: 26963674
    28. two conserved transcription regulators essential for the longevity of germline-less adults, DAF-16/FOXO3A and TCER-1/TCERG1, concurrently enhance the expression of multiple genes involved in lipid synthesis and breakdown and promote longevity. PMID: 26862916
    29. Results report a new component of the insulin/IGF-1 signaling longevity pathway, SMK-1, which specifically influences DAF-16-dependent regulation of the aging process in C. elegans by regulating the transcriptional specificity of DAF-16 activity. PMID: 16530049
    30. this studies suggesting that DAF-16F plays a more prominent role in life span control than DAF-16A, isoform-specific daf-16/FoxO mutant phenotypes and whole transcriptome profiling revealed a predominant role for DAF-16A over DAF-16F in life span control PMID: 26219299
    31. The dbl-1/TGF-beta and daf-12/NHR pathways have not previously been shown to affect L1 development, but disruption of these pathways delayed L1 development in fed larvae, consistent with these pathways promoting development in starved daf-16/FOXO mutants. PMID: 26656736
    32. These results imply that (1) longevity pathways can regulate aging stem cells through various mechanisms, (2) stem cell maintenance is not necessarily prioritized and (3) stem cell regulation can occur at the level of an entire organ system. PMID: 25960195
    33. The DAF-16 regulates natc-1 to modulate stress resistance in Caenorhabditis elegans, linking insulin/IGF-1 signaling to protein N-terminal acetylation. PMID: 25330323
    34. Our mechanistic studies using daf-16 loss-of-function mutant strains (GR1307) indicated that the extension of lifespan by OA requires daf-16. PMID: 26592451
    35. The Deubiquitylase MATH-33 stabilizes DAF-16 protein levels in Insulin/IGF-1 signaling. PMID: 26154057
    36. together, neuron-specific and canonical insulin/insulin-like growth factor signalling/FOXO-regulated targets enable the coordinated extension of neuronal activities, metabolism, and longevity under low-insulin signalling conditions PMID: 26675724
    37. the RNA helicase HEL-1 appears to promote longevity in response to decreased insulin/insulin-like growth factor 1 (IGF-1) signaling as a transcription coregulator of DAF-16. PMID: 26195740
    38. DAF-16 activation bypasses ire-1 requirement for endoplasmic reticulum homeostasis and function. PMID: 25448701
    39. antioxidant treatment can extend the lifespan of Caenorhabditis elegans (C. elegans) through the nuclear translocation of the forkhead box O transcription factor (FoxO) homolog DAF-16. PMID: 24123695
    40. Data show the function of membrane glycoprotein ida-1 as a modulator in insulin receptor Daf-2/transcription factor Daf-16 insulin like signalling pathway thus possibly being a common link between Parkinson's disease and diabetes. PMID: 25469508
    41. phosphorylation of DAF-16 at S286 by UNC-43 is removed by TAX-6*CNB-1, leading to DAF-16 inactivation PMID: 23805378
    42. Reduced insulin/IGF-1 signalling can promote C. elegans longevity through a program that is genetically distinct from the dauer pathway, and requires the Nrf (NF-E2-related factor) orthologue SKN-1 acting in parallel to DAF-16 PMID: 25517099
    43. the FOXO transcription factor DAF-16 is activated in response to DNA damage during development, whereas the DNA damage responsiveness of DAF-16 declines with ageing. PMID: 25419847
    44. the expression of ins-7, a DAF-16 target gene, was abnormally regulated by celecoxib. PMID: 24945567
    45. Efl-1 regulates organismal senescence by inhibiting daf-16. PMID: 25344604
    46. Using LC-MS/MS-based proteomics analysis, we found that activation of DAF-16 in the nucleus results in overrepresentation of many enzymes of major, often reciprocally regulated, metabolic pathways. PMID: 24555535
    47. These findings reveal key features of the architecture of the gene-regulatory network centered on DAF-16, and raise the possibility that activation of AMP-independent AMPK in nutritionally replete daf-2 mutant adults slows aging in C. elegans PMID: 24516399
    48. mdl-1 is a direct transcriptional target of DAF-16. PMID: 24531613
    49. FoxO/Daf-16 removes heat stress damage and restores movement via inhibition of the insulin-like signaling pathway in C. elegans, suggesting that FoxO/Daf-16 plays a critical role in thermotolerance. PMID: 24472780
    50. Thus, the EGL-30-Ca(2+)-BLI-3-CST-1-DAF-16 signaling represents a previously unknown pathway to regulate epidermal damage response. PMID: 24146615

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  • 亚细胞定位：
    Nucleus. Cytoplasm.
  • 组织特异性：
    Isoform b and isoform c are expressed in ectoderm, muscles, intestine and neurons. Isoform b is also expressed in the pharynx. The intestine appears to be the primary site of longevity function.
  • 数据库链接：

    KEGG: cel:CELE_R13H8.1

    STRING: 6239.R13H8.1h

    UniGene: Cel.18378

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