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Recombinant Escherichia phage MS2 Capsid protein

  • 中文名称:
    Recombinant Escherichia phage MS2 Capsid protein
  • 货号:
    CSB-EP365998ECZa2
  • 规格:
    ¥2328
  • 图片:
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他:

产品详情

  • 纯度:
    Greater than 90% as determined by SDS-PAGE.
  • 生物活性:
    Not Test
  • 基因名:
    N/A
  • Uniprot No.:
  • 别名:
    CP;Coat protein
  • 种属:
    Escherichia phage MS2 (Bacteriophage MS2)
  • 蛋白长度:
    Full Length of Mature Protein
  • 来源:
    E.coli
  • 分子量:
    26.7 kDa
  • 表达区域:
    2-130aa
  • 氨基酸序列
    ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVTCSVRQSSAQNRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATNSDCELIVKAMQGLLKDGNPIPSAIAANSGIY
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签:
    N-terminal 6xHis-SUMO-tagged
  • 产品提供形式:
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液:
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
  • 复溶:
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件:
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期:
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期:
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事项:
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA:
    Please contact us to get it.

产品评价

靶点详情

  • 功能:
    Capsid protein self-assembles to form an icosahedral capsid with a T=3 symmetry, about 26 nm in diameter, and consisting of 89 capsid proteins dimers (178 capsid proteins). Involved in viral genome encapsidation through the interaction between a capsid protein dimer and the multiple packaging signals present in the RNA genome. The capsid contains also 1 copy of the A2 maturation protein.; Acts as a translational repressor of viral replicase synthesis late in infection. This latter function is the result of capsid protein interaction with an RNA hairpin which contains the replicase ribosome-binding site.
  • 基因功能参考文献:
    1. When the MS2 capsid assembles, the conformation of bound RNA and its interaction with the PRR1 coat-protein dimer are similar in both small RNA phages PRR1 and phage MS2. PMID: 23519411
    2. A new concept in virus biology provides predictive information on structural constraints on coat protein and genome topography, revealing a previously unrecognized structural interdependence of the shapes and sizes of different viral components. PMID: 23403965
    3. This study shows that translational repressor binding results in self-association of asymmetric (AB) dimers being inhibited, whilst association of AB with symmetric (CC) dimers is greatly enhanced. PMID: 20562027
    4. MS2 coat protein binds several Escherichia coli mRNAs in vitro PMID: 11058143
  • 亚细胞定位:
    Virion.
  • 蛋白家族:
    Levivirus capsid protein family
  • 数据库链接:

    KEGG: vg:1260899