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  4. Recombinant Escherichia coli Transcription-repair-coupling factor (mfd), partial

Recombinant Escherichia coli Transcription-repair-coupling factor (mfd), partial

  • 中文名称：
    大肠杆菌mfd重组蛋白
  • 货号：
    CSB-YP329031ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌mfd重组蛋白
  • 货号：
    CSB-EP329031ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌mfd重组蛋白
  • 货号：
    CSB-EP329031ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌mfd重组蛋白
  • 货号：
    CSB-BP329031ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌mfd重组蛋白
  • 货号：
    CSB-MP329031ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    mfd
  • Uniprot No.：
    P30958
  • 别名：
    mfd; b1114; JW1100Transcription-repair-coupling factor; TRCF; EC 3.6.4.-
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Couples transcription and DNA repair by recognizing RNA polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent release of RNAP and its truncated transcript from the DNA, and recruitment of nucleotide excision repair machinery to the damaged site. Can also dissociate RNAP that is blocked by low concentration of nucleoside triphosphates or by physical obstruction, such as bound proteins. In addition, can rescue arrested complexes by promoting forward translocation. Has ATPase activity, which is required for removal of stalled RNAP, but seems to lack helicase activity. May act through a translocase activity that rewinds upstream DNA, leading either to translocation or to release of RNAP when the enzyme active site cannot continue elongation.
  • 基因功能参考文献：
    1. The results reveal that bacterial RNA polymerase is frequently rescued by Mfd during normal growth and highlight a ubiquitous house-keeping role for Mfd in regulating transcription elongation. PMID: 29679003
    2. Mfd, which is an ATP-dependent superfamily 2 helicase that binds to RNAP, continues to translocate along DNA after RNAP has been displaced until a lesion in the template strand is located. PMID: 24554077
    3. TRCF derepression is associated with profound conformational changes that primarily involve a reorganization of the interdomain interactions. PMID: 21185303
    4. Mfd might form a excinuclease ABC subunit A recruitment factor at stalled transcription complexes that architecturally PMID: 16309703
    5. preliminary structure determination of Escherichia coli Mfd, the transcription-repair coupling factor PMID: 16511235

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    UvrB family; Helicase family, RecG subfamily
  • 数据库链接：

    KEGG: ecj:JW1100

    STRING: 316385.ECDH10B_1186

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