Recombinant Escherichia coli Superoxide dismutase [Mn] (sodA)

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中文名称：

Recombinant Escherichia coli Superoxide dismutase [Mn] (sodA)
货号：

CSB-EP022398ENV
规格：

￥1536
图片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

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纯度：

Greater than 95% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

sodA
Uniprot No.：

P00448
别名：

MnSOD
种属：

Escherichia coli (strain K12)
蛋白长度：

Full Length of Mature Protein
来源：

E.coli
分子量：

23.9 kDa
表达区域：

2-206aa
氨基酸序列

SYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALESLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAAIERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGASGFPIMGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
蛋白标签：

C-terminal 6xHis-tagged
产品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
缓冲液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
复溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
储存条件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保质期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
货期：

3-7 business days
注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

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功能：

Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
基因功能参考文献：
1. WT-MnSOD protein conserves a destabilizing amino acid at position 146 as part of a strategy to favor metal ion binding. PMID: 28704037
2. Escherichia coli manganese superoxide dismutase has two exponential kinetic processes (fast and slow phases) associated with metal binding PMID: 16258041
3. emphasize the role of residue 66 in catalysis and inhibition and provide a structural explanation for differences in catalytic properties between human and certain bacterial forms of manganese superoxide dismutases PMID: 18044968
蛋白家族：

Iron/manganese superoxide dismutase family
数据库链接：

KEGG: ecj:JW3879

STRING: 316385.ECDH10B_4098

Recombinant Escherichia coli Superoxide dismutase [Mn] (sodA)

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