Recombinant Escherichia coli RNA polymerase sigma factor for flagellar operon (fliA)

Greater than 85% as determined by SDS-PAGE.

fliA

P0AEM6

RNA polymerase sigma factor for flagellar operon Sigma F Sigma-27 Sigma-28

Escherichia coli (strain K12)

Full Length

E.coli

31.6 kDa

1-239aa

N-terminal 6xHis-tagged

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

3-7 business days

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Please contact us to get it.