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  4. Recombinant Escherichia coli RNA-binding protein Hfq (hfq)

Recombinant Escherichia coli RNA-binding protein Hfq (hfq)

In Stock
  • 中文名称：
    大肠杆菌hfq重组蛋白
  • 货号：
    CSB-EP363917ENV
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 85% as determined by SDS-PAGE.
  • 基因名：
    hfq
  • Uniprot No.：
    P0A6X3
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Full Length of Mature Protein
  • 来源：
    E.coli
  • 分子量：
    18.5 kDa
  • 表达区域：
    2-102aa
  • 氨基酸序列
    AKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVILLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 10xHis-tagged and C-terminal Myc-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    3-7 business days
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

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hfq Proteins

靶点详情

  • 功能：
    RNA chaperone that binds small regulatory RNA (sRNAs) and mRNAs to facilitate mRNA translational regulation in response to envelope stress, environmental stress and changes in metabolite concentrations. Involved in the regulation of stress responses mediated by the sigma factors RpoS, sigma-E and sigma-32. Binds with high specificity to tRNAs. Binds sRNA antitoxin RalA. In vitro, stimulates synthesis of long tails by poly(A) polymerase I. Required for RNA phage Qbeta replication. Seems to play a role in persister cell formation; upon overexpression decreases persister cell formation while deletion increases persister formation.
  • 基因功能参考文献：
    1. By interacting with the mutS leader, Hfq serves as a critical switch that modulates bacteria from high-fidelity DNA replication to stress-induced mutagenesis PMID: 28794186
    2. Here, de novo Rosetta modeling and competitive binding experiments show that the acidic tip of the Escherichia coli Hfq CTD transiently binds the basic Sm core residues necessary for RNA annealing. PMID: 28826489
    3. Compaction and condensation of DNA mediated by the C-terminal domain of Hfq has been reported. PMID: 28521053
    4. Global mapping of small RNA-Hfq target interactions have been described in the bacterial genome. PMID: 27588604
    5. We describe a refined system - consisting solely of purified Hfq, polynucleotide phosporylase (PNP) and ADP - that allows reconstitution in vitro of characteristic 'SDS-insensitive' Hfq6-Hfq6 assemblies observed in experiments with whole-cell extracts obtained from exponentially-growing cells. We also optimized conditions for the extraction of intact native dodecameric Hfq complexes. PMID: 28366634
    6. The 3' terminal oligo(U) stretch and the base-pairing region enable small RNA to interact with Hfq. PMID: 26531825
    7. Hfq has at least two distinct ways in which it interacts with sRNAs; these different binding properties have strong effects on the stability of the sRNA in vivo and the sequence requirements of regulated mRNAs. PMID: 26373314
    8. The authors show that an interaction between the distal surface of Hfq and the ribosome-binding site of IS10 transposase mRNA (RNA-IN) is required for repressing translation initiation of the transposase. PMID: 25649688
    9. This study demonstrated that random-order binding of Hfq to sRNA and mRNA in each pair can cause high Hfq concentrations to suppress rather than promote sRNA activity in Escherichia coli. PMID: 26261213
    10. Study shows that Hfq does not affect the transcriptional readthrough event itself; also find that the readthrough products no longer bind to Hfq in vivo. PMID: 26106215
    11. these results suggest that Hfq could function as an anchor/coupling factor responsible for de-solubilization of RNA and its tethering to the degradosome complex. PMID: 25896386
    12. The study verified that the A-rich linker region of OxyS sRNA binds Hfq at its distal side. PMID: 25670676
    13. Data show that the mRNA wraps entirely around the host Ffactor 1 protein Hfq protein, specifically contacting both surfaces. PMID: 25404287
    14. The data indicated that the simultaneous binding to the opposite faces of Hfq was enabled by separate adenosine-rich and uridine-rich sequences in the long, single-stranded region of ChiX. PMID: 25582129
    15. Identification of RNA binding sites of Hfq. PMID: 24288369
    16. Hfq acts as the repressor of cirA mRNA translation in the absence of sRNA. PMID: 24065131
    17. Based on the results presented here, we suggest that Hfq could be involved in regulating RNA pairing in other antisense systems, including systems encoded by other transposable elements. PMID: 23510801
    18. Thus, Hfq is intrinsically essential to pathogenic Escherichia coli for survival and virulence exerted in the Caenorhabditis elegans host. PMID: 22713744
    19. The authors show that Hfq is most active in RNA annealing when the hexamer is present. PMID: 22326348
    20. sRNAs compete with each other for Hfq binding. PMID: 22040174
    21. expression of hfq was repressed by the CRP-cAMP complex. PMID: 21840983
    22. the purification, crystallization and structural data of the full 102-amino-acid protein are presented. These data revealed that the presence of the C-terminus changes the crystal packing of E. coli Hfq PMID: 21543856
    23. Results revealed that the C-terminus of Hfq is intrinsically disordered and flexible, which appears to contribute to the interactions of Hfq with RNA substrates. PMID: 21330354
    24. role of Hfq in LF82 virulence was independent of its function in regulating RpoS and RpoE activity PMID: 21298102
    25. Hfq negatively regulate Tn10/IS10 transposition by promoting anti-sense inhibition of RNA-IN10 translation. PMID: 20815820
    26. Hfq mediates the binding of sRNAs to their target mRNAs; without Hfq, most sRNAs cannot efficiently regulate target mRNA expression. PMID: 21189298
    27. examined the ability of Hfq derivatives, consisting of the conserved core and short C-terminal extensions, to support the regulation of rpoS expression and riboregulation by various well-characterized small regulatory RNAs PMID: 20888338
    28. LEE regulator (GrlA) is critically involved in the regulating the presence of L0045. PMID: 20587027
    29. show that both Hfq and this Hfq binding site are required for RprA or ArcZ to act in vivo and to form a stable complex with rpoS mRNA in vitro PMID: 20457943
    30. The alteration of rpsO, rpsT and rpsB-tsf transcript levels observed under conditions of Hfq deficiency is not caused by the post-transcriptional events. PMID: 20167073
    31. Hfq has two independent RNA-binding surfaces; Hfq can simultaneously bind a ncRNA and its mRNA target to facilitate the strand displacement reaction required for Hfq-dependent translational regulation PMID: 15531892
    32. Hfq synthesis is autoregulated at the translational level. PMID: 15872186
    33. E.coli Hfq polymerizes into well-ordered fibres whose morphologies closely resemble those found for Sm-like archaeal proteins PMID: 16337963
    34. findings indicate that Hfq regulates traM and traJ transcript stability by a mechanism separate from FinOP-mediated repression PMID: 16352828
    35. Study shows that SgrS RNA forms a stable duplex with the phosphoenolpyruvate-glucose phosphotransferase mRNA, and that Hfq markedly facilitates the rate of duplex formation. PMID: 16859494
    36. Hfq may play a general role in stress response regulation in E. coli. PMID: 17158661
    37. Hfq modulates the interaction between a small non-coding RNA, DsrA, and its regulatory target mRNA, rpoS. PMID: 17259214
    38. structure and function of Hfq [review] PMID: 17395525
    39. The increase of the CCA-addition in the presence of Hfq suggests that this protein acts as a stimulating factor not only for PAP, but also for the CCA-adding enzyme. PMID: 17949481
    40. The C-terminal extension of E. coli Hfq constitutes a hitherto unrecognized RNA interaction surface with specificity for mRNAs. PMID: 18000007
    41. tRNA binding and reduced fidelity are linked by a role for Hfq in tRNA modification. PMID: 18230766
    42. Hfq negatively affects LEE expression in exponential phase by regulating the expression of the LEE-encoded regulators GrlA and GrlR at the post-transcriptional level by destabilizing grlRA mRNA. PMID: 19570135
    43. HFq protein is involved in the regulation of bacterial cytoskeleton and cell division proteins. PMID: 19571680
    44. Study showed a novel property of Escherichia coli Hfq protein: it possibly influenced extracellular indole levels. PMID: 19657572
    45. the crystal structure of Escherichia coli Hfq bound to the poly(A) RNA, A(15) is reported. PMID: 19889981
    46. Hfq binds with high specificity and nanomolar affinity to both D-stem and T-stem of tRNAs using its proximal surface. PMID: 18230766

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  • 蛋白家族：
    Hfq family
  • 数据库链接：

    KEGG: ecj:JW4130

    STRING: 316385.ECDH10B_4367

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