Recombinant Escherichia coli Nickel-responsive regulator (nikR)

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中文名称：

大肠杆菌nikR重组蛋白
货号：

CSB-EP363929ENV
规格：

￥2328
图片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

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纯度：

Greater than 90% as determined by SDS-PAGE.
基因名：

nikR
Uniprot No.：

P0A6Z6
别名：

nikR; yhhG; b3481; JW3446Nickel-responsive regulator
种属：

Escherichia coli (strain K12)
蛋白长度：

Full Length
来源：

E.coli
分子量：

19.1 kDa
表达区域：

1-133aa
氨基酸序列

MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEATQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLKGDMGDVQHFADDVIAQRGVRHGHLQCLPKED
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
蛋白标签：

N-terminal 6xHis-tagged
产品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
缓冲液：

Tris-based buffer,50% glycerol
储存条件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保质期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
货期：

3-7 business days
注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

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功能：

Transcriptional repressor of the nikABCDE operon. Is active in the presence of excessive concentrations of intracellular nickel.
基因功能参考文献：
1. Structural basis of low-affinity nickel binding to the nickel-responsive transcription factor NikR from Escherichia coli PMID: 20704276
2. localized this Ni(II) site to a region at the interface between the metal- and DNA-binding domains and identified His48 and His110 as residues that participate in the low-affinity Ni(II)-binding response PMID: 20583753
3. Mutagenesis and cation exchange experiments reveal that K(+) is a critical structural component for the activation of Ni(II)-responsive DNA binding by NikR. PMID: 20088519
4. This data sheds light on the Ni(II)-selective conformational changes that allow NikR to bind DNA optimally and repress transcription of the nik operon. PMID: 16133200
5. Two crystal structures of nickel-activated E. coli NikR were reported. PMID: 16945905
6. NikR response is specific to nickel in vivo. PMID: 17397155
7. Results examine a series of residue interrelationships that describe an allosteric communication pathway between the Ni(2+)- and DNA-binding sites of NikR, which are separated by 40 A. PMID: 18433769
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蛋白家族：

Transcriptional regulatory CopG/NikR family
数据库链接：

KEGG: ecj:JW3446

STRING: 316385.ECDH10B_3655

Recombinant Escherichia coli Nickel-responsive regulator (nikR)

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