Recombinant Escherichia coli NADPH-dependent ferric-chelate reductase (yqjH)

^{In Stock}

中文名称：

大肠杆菌yqjH重组蛋白
货号：

CSB-EP671608ENV
规格：

￥2328
图片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

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纯度：

Greater than 95% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

yqjH
Uniprot No.：

Q46871
别名：

Ferric siderophore reductase
种属：

Escherichia coli (strain K12)
蛋白长度：

Full Length
来源：

E.coli
分子量：

35.8 kDa
表达区域：

1-254aa
氨基酸序列

MNNTPRYPQRVRNDLRFRELTVLRVERISAGFQRIVLGGEALDGFTSRGFDDHSKLFFPQPDAHFVPPTVTEEGIVWPEGPRPPSRDYTPLYDELRHELAIDFFIHDGGVASGWAMQAQPGDKLTVAGPRGSLVVPEDYAYQLYVCDESGMPALRRRLETLSKLAVKPQVSALVSVRDNACQDYLAHLDGFNIEWLAHDEQAVDARLAQMQIPADDYFIWITGEGKVVKNLSRRFEAEQYDPQRVRAAAYWHAK
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
蛋白标签：

C-terminal 6xHis-tagged
产品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
缓冲液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
复溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
储存条件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保质期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
货期：

3-7 business days
注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

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功能：

Plays a role in iron homeostasis under excess nickel conditions.
基因功能参考文献：
1. Site-directed mutagenesis reveals that residues K55 and R130 are crucial for substrate binding and ferric reductase activity. Catalytic/structural analysis: substrate specificity, kinetic analysis, reaction mechanism, amino acid sequence alignment. PMID: 22098718
2. The authors show that YqjH has ferric reductase activity and is required for iron homeostasis in Escherichia coli. PMID: 21097627
亚细胞定位：

Cytoplasm.
蛋白家族：

SIP oxidoreductase family
数据库链接：

KEGG: ecj:JW3041

STRING: 316385.ECDH10B_3245

Recombinant Escherichia coli NADPH-dependent ferric-chelate reductase (yqjH)

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