Recombinant Escherichia coli NAD-dependent protein deacylase (cobB)

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中文名称：

Recombinant Escherichia coli NAD-dependent protein deacylase(cobB)
货号：

CSB-EP304314ENV
规格：

￥2328
图片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

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纯度：

Greater than 95% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

cobB
Uniprot No.：

P75960
别名：

(Regulatory protein SIR2 homolog)
种属：

Escherichia coli (strain K12)
蛋白长度：

Full Length of Isoform 2
来源：

E.coli
分子量：

34.3
表达区域：

1-242aa
氨基酸序列

MEKPRVLVLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQPEIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQVLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADIFIAIGTSGHVYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLKGLKAGSIA
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
蛋白标签：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
产品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
缓冲液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
复溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
储存条件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保质期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
货期：

3-7 business days
注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

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功能：

NAD-dependent lysine deacetylase and desuccinylase that specifically removes acetyl and succinyl groups on target proteins. Modulates the activities of several proteins which are inactive in their acylated form. Activates the enzyme acetyl-CoA synthetase by deacetylating 'Lys-609' in the inactive, acetylated form of the enzyme. May also modulate the activity of other propionyl-adenosine monophosphate (AMP)-forming enzymes.
基因功能参考文献：
1. discovered that CobB acts as a lipoamidase; results indicate that CobB has the ability to regulate pyruvate dehydrogenase and alpha-ketoglutarate dehydrogenase activity under different growth conditions PMID: 28900027
2. CobB can reverse acetylation regardless of acetyl donor or acetylation mechanism. PMID: 25417765
3. CobB is a bifunctional enzyme with lysine desuccinylation and deacetylation activities. PMID: 24176774
4. CobB play crucial roles in the transcription regulation of the periplasmic stress-responsive promoter cpxP when cells of Escherichia coli grow in the presence of glucose, an environment that induces protein acetylation PMID: 21696463
5. Results suggest that CobB regulates E. coli chemotaxis by deacetylating CheY. PMID: 20345663
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亚细胞定位：

Cytoplasm.
蛋白家族：

Sirtuin family, Class III subfamily
数据库链接：

KEGG: ecj:JW1106

STRING: 316385.ECDH10B_1192

Recombinant Escherichia coli NAD-dependent protein deacylase (cobB)

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