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  4. Recombinant Escherichia coli Formamidopyrimidine-DNA glycosylase (mutM)

Recombinant Escherichia coli Formamidopyrimidine-DNA glycosylase (mutM)

  • 中文名称：
    Recombinant Escherichia coli Formamidopyrimidine-DNA glycosylase (mutM)
  • 货号：
    CSB-EP361544ENV
  • 规格：
    ￥1536
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 95% as determined by SDS-PAGE.
  • 生物活性：
    Not Test
  • 基因名：
    mutM
  • Uniprot No.：
    P05523
  • 别名：
    Fapy-DNA glycosylase;DNA-apurinic or apyrimidinic site;AP lyase MutM
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Full Length
  • 来源：
    E.coli
  • 分子量：
    37.2 kDa
  • 表达区域：
    1-269aa
  • 氨基酸序列
    MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRLSDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKVLRYTDPRRFGAWLWTKELEGHNVLTHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMDNKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLKDFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    C-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

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mutM Proteins

靶点详情

  • 功能：
    Involved in base excision repair of DNA damaged by oxidation or by mutagenic agents. Acts as DNA glycosylase that recognizes and removes damaged bases. Has a preference for oxidized purines, such as 7,8-dihydro-8-oxoguanine (8-oxoG) and its derivatives such as guanidinohydantoin:C and spiroiminodihydantoin:C, however it also acts on thymine glycol:G, 5,6-dihydrouracil:G and 5-hydroxyuracil:G. Has AP (apurinic/apyrimidinic) lyase activity and introduces nicks in the DNA strand. Cleaves the DNA backbone by beta-delta elimination to generate a single-strand break at the site of the removed base with both 3'- and 5'-phosphates. Cleaves ssDNA containing an AP site.
  • 基因功能参考文献：
    1. expression of the bacterial DNA repair protein FPG stably protects human lung cells from the mutagenic effects of CS by improving cells' capacity to repair damaged DNA. PMID: 24498234
    2. Structural and biochemical analysis of DNA helix invasion by the bacterial 8-oxoguanine DNA glycosylase MutM PMID: 23404556
    3. Fpg protein seemed to be very important in the repair of ultraviolet-C induced DNA lesions. PMID: 22732937
    4. structures reveal that MutM imposes the same extrusion-prone backbone conformation on the oxoG lesion irrespective of its 5'-neighbor while leaving the rest of the DNA relatively free to adjust to the particular demands of individual sequences PMID: 22465958
    5. demonstrates that it is feasible to increase resistance of human TM cell to endogenous oxidative damage by FPG gene transfection PMID: 21561236
    6. Over-expressed, purified and characterized two stable isotope-labeled DNA glycosylases, i.e., (15)N-labeled Escherichia coli formamidopyrimidine DNA glycosylase (Fpg) and (15)N-labeled human 8-oxoguanine-DNA glycosylase (hOGG1). PMID: 21356311
    7. examination of the structural and dynamic changes that occur in solution when Fpg binds DNA PMID: 15661656
    8. A base excision repair enzyme involved in the 8-oxoguanine (oxoG) repair pathway. PMID: 15979229
    9. when the enzyme encounters its true substrate, 8-oxoG.C, the complex enters the productive catalytic reaction after approximately 50 ms, partitioning the substrate away from the competing dissociation process PMID: 17209553
    10. The substrate specificity of two similar enzymes, MutM of E. coli and OGG1 of H. sapiens, is reported. PMID: 17536801
    11. The high affinity and efficient activity of EcFpg toward the hydantoin lesions suggest that EcFpg mediates repair of the lesions in vivo. PMID: 17655276
    12. Data show a lack of interaction of mutM with dnaX36 since the double dnaX36 mutM strains did not show any major changes in the frequency of G.CT.A transversions compared to the single dnaX36 strain. PMID: 18156258
    13. analysis of the the driving forces that dictate Fpg enzyme efficiency and specificity, which helps to elucidate the energy landscape for lesion recognition and repair PMID: 18172202
    14. Data show that processive cleavage by Fpg does not correlate with their substrate specificity and under nearly physiological salt conditions may be replaced with the distributive mode of action. PMID: 18672903

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  • 蛋白家族：
    FPG family
  • 数据库链接：

    KEGG: ecj:JW3610

    STRING: 316385.ECDH10B_3817

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