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  4. Recombinant Escherichia coli Bifunctional protein putA (putA), partial

Recombinant Escherichia coli Bifunctional protein putA (putA), partial

  • 中文名称：
    大肠杆菌putA重组蛋白
  • 货号：
    CSB-YP357703ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    大肠杆菌putA重组蛋白
  • 货号：
    CSB-EP357703ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    大肠杆菌putA重组蛋白
  • 货号：
    CSB-EP357703ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    大肠杆菌putA重组蛋白
  • 货号：
    CSB-BP357703ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    大肠杆菌putA重组蛋白
  • 货号：
    CSB-MP357703ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    putA
  • Uniprot No.：
    P09546
  • 别名：
    putA; poaA; b1014; JW0999; Bifunctional protein PutA [Includes: Proline dehydrogenase; EC 1.5.5.2; Proline oxidase); Delta-1-pyrroline-5-carboxylate dehydrogenase; P5C dehydrogenase; EC 1.2.1.88; L-glutamate gamma-semialdehyde dehydrogenase)]
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Oxidizes proline to glutamate for use as a carbon and nitrogen source and also function as a transcriptional repressor of the put operon.
  • 基因功能参考文献：
    1. Deletion of the C-terminal motif (CCM) or replacement of hydrophobic residues with negatively charged residues within the CCM impairs EcPutA functional and physical membrane association PMID: 29090935
    2. crystal structures of the proline dehydrogenase domain mutants PutA86-630D370N and PutA86-630D370A complexed with the proline analogue show that the mutations cause only minor perturbations to the active site but no major structural changes PMID: 23713611
    3. Data show that PutA is a symmetric V-shaped dimer having dimensions of 205 x 85 x 55 A. The particle consists of two large lobes connected by a 30-A diameter cylinder PMID: 22013066
    4. kinetic data, along with analysis of crystal structure data for the PRODH domain, suggest that the proline:ubiquinone oxidoreductase reaction occurs via a rapid equilibrium ping-pong mechanism with proline and ubiquinone binding at two distinct sites PMID: 22040654
    5. The proline dehydrogenase (PRODH)/L-tetrahydro-2-furoic acid complex is the first structure of PRODH with a five-membered ring proline analogue bound in the active site. PMID: 15449943
    6. From this study it is clear that reduction of flavin adenine dinucleotide bound to the proline dehydrogenase active site of PutA elicits global conforamtional changes that direct PutA-membrane associations. PMID: 15476410
    7. identify molecular interactions in the PutA active site that underlie redox-dependent functional switching of PutA PMID: 17209558
    8. analysis of metabolism and gene expression in the proline utilization A protein from Escherichia coli [review] PMID: 18324349

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  • 蛋白家族：
    Proline dehydrogenase family; Aldehyde dehydrogenase family
  • 数据库链接：

    KEGG: ecj:JW0999

    STRING: 316385.ECDH10B_1086

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