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  4. Recombinant Escherichia coli Alanine--tRNA ligase (alaS), partial

Recombinant Escherichia coli Alanine--tRNA ligase (alaS), partial

  • 中文名称：
    Recombinant Escherichia coli Alanine--tRNA ligase(alaS) ,partial
  • 货号：
    CSB-YP360465ENV
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Escherichia coli Alanine--tRNA ligase(alaS) ,partial
  • 货号：
    CSB-EP360465ENV
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Escherichia coli Alanine--tRNA ligase(alaS) ,partial
  • 货号：
    CSB-EP360465ENV-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Escherichia coli Alanine--tRNA ligase(alaS) ,partial
  • 货号：
    CSB-BP360465ENV
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Escherichia coli Alanine--tRNA ligase(alaS) ,partial
  • 货号：
    CSB-MP360465ENV
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    alaS
  • Uniprot No.：
    P00957
  • 别名：
    alaS; lovB; b2697; JW2667; Alanine--tRNA ligase; EC 6.1.1.7; Alanyl-tRNA synthetase; AlaRS
  • 种属：
    Escherichia coli (strain K12)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalyzes the attachment of L-alanine to tRNA(Ala) in a two-step reaction: L-alanine is first activated by ATP to form Ala-AMP and then transferred to the acceptor end of tRNA(Ala). AlaRS also incorrectly activates the sterically smaller amino acid glycine as well as the sterically larger amino acid L-serine; generates 2-fold more mischarged Gly than Ser. These mischarged amino acids occur because the of inherent physicochemical limitations on discrimination between closely related amino acids (Ala, Gly and Ser) in the charging step.; Edits mischarged Ser-tRNA(Ala) and Gly-tRNA(Ala) but not incorrectly charged Ser-tRNA(Thr). Dtd edits Gly-tRNA(Ala) 4-fold better than does AlaRS.; Attaches Ala to transfer-messenger RNA (tmRNA, also known as 10Sa RNA, the product of the ssrA gene). tmRNA plays a major role in rescue of stalled ribosomes via trans-translation.
  • 基因功能参考文献：
    1. Here, the authors show that DTD's active site architecture can efficiently edit mischarged Gly-tRNA(Ala) species four orders of magnitude more efficiently than even AlaRS, the only ubiquitous cellular checkpoint known for clearing the error. PMID: 28362257
    2. ATP binding to full length enzyme (ARS875) and to an N-terminal construct (ARS461) is endothermic (DeltaH = 3-4 kcal mol(-1)) with stoichiometries of 1:1 for ARS461 and 2:1 for full-length dimer. PMID: 21985608
    3. the editing site of alanyl-tRNA synthetase targets mischarged tRNA(Ala) using a structural motif unrelated to that for aminoacylation, so that, remarkably, two motifs in the same enzyme independently provide determinants for tRNA(Ala) recognition PMID: 18172502
    4. findings show that the C-Ala domain plays a key role in alanyl-tRNA synthetases (AlaRS) function; C-Ala forms an ancient single-stranded nucleic acid binding motif that promotes cooperative binding of both aminoacylation and editing domains to tRNA(Ala) PMID: 19661429
  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Class-II aminoacyl-tRNA synthetase family
  • 数据库链接：

    KEGG: ecj:JW2667

    STRING: 316385.ECDH10B_2865

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