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  4. Recombinant Enterobacteria phage lambda Terminase small subunit (Nu1)

Recombinant Enterobacteria phage lambda Terminase small subunit (Nu1)

  • 中文名称：
    Recombinant Enterobacteria phage lambda Terminase small subunit(Nu1)
  • 货号：
    CSB-YP366025ECW
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Enterobacteria phage lambda Terminase small subunit(Nu1)
  • 货号：
    CSB-EP366025ECW
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Enterobacteria phage lambda Terminase small subunit(Nu1)
  • 货号：
    CSB-EP366025ECW-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Enterobacteria phage lambda Terminase small subunit(Nu1)
  • 货号：
    CSB-BP366025ECW
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Enterobacteria phage lambda Terminase small subunit(Nu1)
  • 货号：
    CSB-MP366025ECW
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Nu1
  • Uniprot No.：
    P03707
  • 别名：
    Nu1; lambdap01; Terminase small subunit; EC 3.6.4.-; DNA-packaging protein Nu1; Gene product Nu1; gpNu1
  • 种属：
    Enterobacteria phage lambda (Bacteriophage lambda)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-181
  • 氨基酸序列
    MEVNKKQLAD IFGASIRTIQ NWQEQGMPVL RGGGKGNEVL YDSAAVIKWY AERDAEIENE KLRREVEELR QASEADLQPG TIEYERHRLT RAQADAQELK NARDSAEVVE TAFCTFVLSR IAGEIASILD GLPLSVQRRF PELENRHVDF LKRDIIKAMN KAAALDELIP GLLSEYIEQS G
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The small subunit is responsible for the binding to multiple recognition elements within the packaging initiation site cos. The terminase lies at a unique vertex of the procapsid and is composed of two subunits, a small terminase subunit involved in viral DNA recognition (binding to packaging sequence cos), and a large terminase subunit possessing endonucleolytic, ATPase and helicase activities (DNA maturation and packaging). The terminase binds, cooperatively with the host factor IHFA/IHFB, to the cos site at the junction of adjacent viral genomes in the concatemeric DNA. The endonuclease and helicase activities of the large subunit cleave the viral DNA generating 5'overhangs of 12 bp in length. The terminase remains bound to the left end of the genome to be packaged, forming a stable DNA-terminase complex. In a reaction facilitated by the viral assembly catalyst gpFI, the DNA-terminase complex binds to the portal of the procapsid and the terminase packages the viral DNA into the procapsid until the next cos site on the concatemer reaches the complex. The downstream cos site is then cut generating the mature right end of the genome, the heterotrimer undocks from the DNA-filled head and remains bound to the left end of concatemer's next genome.
  • 亚细胞定位：
    Host cytoplasm.
  • 蛋白家族：
    Terminase small subunit family
  • 数据库链接：

    KEGG: vg:2703523

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