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  4. Recombinant Escherichia phage T7 DNA primase/helicase (4), partial

Recombinant Escherichia phage T7 DNA primase/helicase (4), partial

  • 中文名称：
    Recombinant Escherichia phage T7 DNA primase/helicase(4),partial
  • 货号：
    CSB-YP356120EEB
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Escherichia phage T7 DNA primase/helicase(4),partial
  • 货号：
    CSB-EP356120EEB-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Escherichia phage T7 DNA primase/helicase(4),partial
  • 货号：
    CSB-BP356120EEB
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Escherichia phage T7 DNA primase/helicase(4),partial
  • 货号：
    CSB-MP356120EEB
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    4
  • Uniprot No.：
    P03692
  • 别名：
    4; DNA primase/helicase; EC 2.7.7.-; EC 3.6.4.12; Gene product 4; Gp4
  • 种属：
    Escherichia phage T7 (Bacteriophage T7)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

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4 Proteins

问答及客户评论

■ 常见问题解答
Q:

Have you received any customer feedback about the activity of this protein?
I'm especially interested in receiving detailed Tag information.

A:
Thanks for your inquiry.
Sorry, as all of them are semi-customized proteins that we haven't expressed before, so the tag type will be determined during the manufacturing process.
However, the expected tag for each protein is list as above, if you have desired tag type or are interested in some other region, pls let us know.
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靶点详情

  • 功能：
    ATP-dependent DNA helicase and primase essential for viral DNA replication and recombination. The helicase moves 5' -> 3' on the lagging strand template, unwinding the DNA duplex ahead of the leading strand polymerase at the replication fork and generating ssDNA for both leading and lagging strand synthesis. ATP or dTTP hydrolysis propels each helicase domain to translocate 2 nt per step sequentially along DNA. Mediates strand transfer when a joint molecule is available and participates in recombinational DNA repair through its role in strand exchange. Primase activity synthesizes short RNA primers at the sequence 5'-GTC-3' on the lagging strand that the polymerase elongates using dNTPs and providing the primase is still present.
  • 基因功能参考文献：
    1. structural model of a bacteriophage T7 DNA helicase-DNA polymerase complex PMID: 22977246
    2. Zinc-binding domain of the bacteriophage T7 DNA primase modulates binding to the DNA template PMID: 23024359
    3. Data show that tryptophans Trp-42, -97, and -147 play different but essential roles in T7 DNA primase. PMID: 22605336
    4. The ligation state of the zinc ion was examined by X-ray absorption spectroscopy and biochemical analysis of genetically altered primases, was analysed. PMID: 19206208
    5. thioredoxin-binding domain of T7 DNA polymerase is the site of interaction of the phage-encoded helicase/primase (gp4) and ssDNA binding protein (gp2.5) PMID: 15795374
    6. residues Glu-157, Glu-159, Asp-161, Asp-207, Asp-209, and Asp-237 are essential for binding affinity for ATP PMID: 15917241
    7. The switch in contacts between Asp(263) and Tyr(411), Lys(408), or Arg(404) in helix E of the adjacent subunit results in conformational changes that modulate helicase and primase activity. PMID: 19574219

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  • 数据库链接：

    KEGG: vg:1261046

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