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  4. Recombinant Enterobacteria phage T4 10 kDa anti-sigma factor (asiA)

Recombinant Enterobacteria phage T4 10 kDa anti-sigma factor (asiA)

  • 中文名称：
    Recombinant Enterobacteria phage T4 10 kDa anti-sigma factor(asiA)
  • 货号：
    CSB-YP327122EDZ
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Enterobacteria phage T4 10 kDa anti-sigma factor(asiA)
  • 货号：
    CSB-EP327122EDZ
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Enterobacteria phage T4 10 kDa anti-sigma factor(asiA)
  • 货号：
    CSB-EP327122EDZ-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Enterobacteria phage T4 10 kDa anti-sigma factor(asiA)
  • 货号：
    CSB-BP327122EDZ
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Enterobacteria phage T4 10 kDa anti-sigma factor(asiA)
  • 货号：
    CSB-MP327122EDZ
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    asiA
  • Uniprot No.：
    P32267
  • 别名：
    asiA; 10 kDa anti-sigma factor; 10 kDa RNA polymerase-associated protein; Audrey Stevens' inhibitor
  • 种属：
    Enterobacteria phage T4 (Bacteriophage T4)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-90
  • 氨基酸序列
    MNKNIDTVRE IITVASILIK FSREDIVENR ANFIAFLNEI GVTHEGRKLN QNSFRKIVSE LTQEDKKTLI DEFNEGFEGV YRYLEMYTNK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcriptional inhibitor. Inhibits sigma 70-directed transcription by weakening its interaction with the core of the host's RNA polymerase. This allows Gp55 to successfully compete for the core enzyme. Plays an important role during the prereplicative period of phage T4 development.
  • 基因功能参考文献：
    1. The authors show that AsiA also plays a direct role in T4 middle promoter activation by contacting the MotA activation domain. PMID: 19843221
    2. AsiA simultaneously occupies the surface of region 4 that ordinarily contacts core RNA polymerase (RNAP) PMID: 15257291
    3. sequence comparisons of T4 AsiA with Rsd, indicate that these proteins share conserved amino acid residues at positions important for the binding of T4 AsiA to sigma70 region 4 PMID: 15561138
    4. AsiA protein plays a key role in T4 development through its ability to both inhibit and activate bacterial RNA polymerase transcription. Review. PMID: 15802215
    5. review summarizes interactions of AsiA and MotA with sigma(70) and discusses how these interactions accomplish the switch to T4 middle promoters [review] PMID: 15941982

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  • 数据库链接：

    KEGG: vg:1258709

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