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  4. Recombinant Drosophila melanogaster Spectrin beta chain (beta-Spec), partial

Recombinant Drosophila melanogaster Spectrin beta chain (beta-Spec), partial

  • 中文名称：
    Recombinant Drosophila melanogaster Spectrin beta chain(beta-Spec),partial
  • 货号：
    CSB-EP251598DLU
  • 规格：
    ￥2328
  • 图片：
    • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • 其他：

产品详情

  • 纯度：
    Greater than 95% as determined by SDS-PAGE.
  • 生物活性：
    Not Test
  • 基因名：
    beta-Spec
  • Uniprot No.：
    Q00963
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    Partial
  • 来源：
    E.coli
  • 分子量：
    35.3
  • 表达区域：
    1-271aa
  • 氨基酸序列
    MTTDISIVRWDPSQGPGNEYIDEYEYDGGNSSSRLFERSRIKALAEERESVQKKTFTKWVNSHLCRVNCRIADLYVDMRDGKHLIKLLEVLSGERLPKPTKGKMRIHCLENVDKALQFLREQRVHLENIGSHDIVDGNASLNLGLIWTIILRFQIQDITIEEVDNKETKSAKDALLLWCQMKTAGYHNVNVRNFTTSWRDGLAFNAIIHKHRPDLVQFEKLSKTNAIHNLNNAFDVAEDKLGLAKLLDAEDVFVEHPDEKSIITYVVTYYH
    Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
  • 蛋白标签：
    N-terminal 6xHis-tagged
  • 产品提供形式：
    Liquid or Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 缓冲液：
    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
  • Datasheet & COA：
    Please contact us to get it.

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beta-Spec antibodies

靶点详情

  • 功能：
    Spectrin is the major constituent of the cytoskeletal network underlying the erythrocyte plasma membrane. It associates with band 4.1 and actin to form the cytoskeletal superstructure of the erythrocyte plasma membrane. Interacts with calmodulin in a calcium-dependent manner.
  • 基因功能参考文献：
    1. The abnormal stress fiber-like actin structure on the basal side of follicle cells provides a likely link between the b-spectrin mutations and the loss of the Hippo signaling activity phenotype. PMID: 25589787
    2. Model in which alphabeta-spectrin directly couples lipid uptake at the plasma membrane to lipid droplet growth in the cytoplasm. In contrast, strong overexpression of beta-spectrin caused fat body atrophy and larval lethality. PMID: 24037266
    3. Data show that DAnk2-binding is critical for beta spectrin function in vivo. PMID: 20573981
    4. Spectrin may be an active partner in the stabilization of polarized membrane domains PMID: 14734656
    5. Presynaptic spectrin functions as an essential presynaptic scaffold that may link synaptic cell adhesion with the stabilization of the underlying microtubule cytoskeleton. PMID: 15916948
    6. Brush border spectrin is required for early endosome recycling. PMID: 16537648
    7. At the Drosophila neuromuscular junction postsynaptic Spectrin-actin lattice acts as an organizing scaffold upon which pre- and postsynaptic development are arranged. PMID: 17088429
    8. beta-Spectrin is required in neurons for proper midline axon guidance in the embryonic CNS. PMID: 17121810

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Cytoplasm, cell cortex.
  • 蛋白家族：
    Spectrin family
  • 数据库链接：

    KEGG: dme:Dmel_CG5870

    STRING: 7227.FBpp0074228

    UniGene: Dm.7022

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