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  4. Recombinant Drosophila melanogaster Protein timeless (tim), partial

Recombinant Drosophila melanogaster Protein timeless (tim), partial

  • 中文名称：
    黑腹果蝇tim重组蛋白
  • 货号：
    CSB-YP342197DLU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    黑腹果蝇tim重组蛋白
  • 货号：
    CSB-EP342197DLU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    黑腹果蝇tim重组蛋白
  • 货号：
    CSB-EP342197DLU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    黑腹果蝇tim重组蛋白
  • 货号：
    CSB-BP342197DLU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    黑腹果蝇tim重组蛋白
  • 货号：
    CSB-MP342197DLU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    tim
  • Uniprot No.：
    P49021
  • 别名：
    tim; CG3234; Protein timeless
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for the production of circadian rhythms. The biological cycle depends on the rhythmic formation and nuclear localization of the TIM-PER complex. Light induces the degradation of TIM, which promotes elimination of PER. Nuclear activity of the heterodimer coordinatively regulates PER and TIM transcription through a negative feedback loop. Behaves as a negative element in circadian transcriptional loop. Does not appear to bind DNA, suggesting indirect transcriptional inhibition.
  • 基因功能参考文献：
    1. data suggest that CRTC is a novel co-activator for the CLK/CYC-activated tim transcription to coordinate molecular rhythms with circadian behaviors over a 24-hour time-scale. PMID: 27577611
    2. A phosphorylation program, native to the master pacemaker neurons, regulates the timing of nuclear accumulation of the Period/Timeless repressor complex. PMID: 27346344
    3. mir-276a inhibits tim expression: Deleting the mir-276a-binding site in the tim 3' UTR causes elevated levels of TIM and approximately 50% arrhythmicity. PMID: 27162360
    4. possible roles it plays in the physiological preservation of health, as well as in the pathogenic mechanisms of related diseases [review] PMID: 26691298
    5. CULLIN-3 is required for the circadian control of PERIOD and TIMELESS oscillations. PMID: 22879814
    6. Nuclear TIM expression is associated with day-night cycles that are independent of the central nervous system. PMID: 22713751
    7. Timeless regulates phagocytosis of bacteria in Drosophila. PMID: 22253593
    8. This study demonstrated that timelss mutant affect TIMELESS and PERIOD post-trnaslational regulation and metabolism in cell nuclear. PMID: 21734289
    9. structural domains PMID: 12372263
    10. Tim1 has a role in sister chromatid cohesion. PMID: 12852847
    11. Regulation of the timeless protein involves nuclear transport mechanisms. PMID: 12944510
    12. A novel tim mutation affects behavioral rhythms but not periodic eclosion. PMID: 15520259
    13. results suggest that JET is essential for resetting the circadian clock by transmitting light signals from cryptochrome to TIMELESS PMID: 16794082
    14. Results suggest that photic stimulation of timeless expression at low temperatures is part of a seasonal adaptive response that helps advance the clock phase on cold days, enabling flies to exhibit daytime activity despite the earlier onset of dusk. PMID: 16864646
    15. Data show that ectopic CRYPTOCHROME renders TIMELESS light-sensitive in the Drosophila ovary. PMID: 16864647
    16. show a strong interaction between Veela and cryptochrome genetic variants, demonstrating that the Jetlag, Timeless, and cryptochrome proteins function in the same pathway PMID: 17068124
    17. study reports that a mutation in the timeless gene has arisen and spread by natural selection relatively recently in Europe; natural and artificial alleles of the timeless gene affect the incidence of diapause in response to changes in light & temperature PMID: 17600215
    18. findings show that the mutant allele attenuates the photosensitivity of the circadian clock and causes decreased dimerization of the mutant TIMELESS protein isoform to CRYPTOCHROME; this interaction results in a more stable TIMELESS product PMID: 17600216
    19. these data indicate that TIM is an important mediator of CK2 effects on circadian rhythms. PMID: 18815259

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  • 亚细胞定位：
    Nucleus. Cytoplasm, perinuclear region. Note=Nuclear at specific periods of the day. First accumulates in the perinuclear region about one hour before translocation into the nucleus. Interaction with per is required for nuclear localization.
  • 蛋白家族：
    Timeless family
  • 组织特异性：
    Expressed in head, photoreceptors, lateral neurons and glial cells in the lamina and medulla of the optic lobes. Expression follows a light-dark cycle, levels show a significant decrease at the end of the night and then remain low throughout the light per
  • 数据库链接：

    KEGG: dme:Dmel_CG3234

    STRING: 7227.FBpp0305454

    UniGene: Dm.2663

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