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  4. Recombinant Drosophila melanogaster Protein smoothened (smo), partial

Recombinant Drosophila melanogaster Protein smoothened (smo), partial

  • 中文名称：
    黑腹果蝇smo重组蛋白
  • 货号：
    CSB-YP309724DLU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    黑腹果蝇smo重组蛋白
  • 货号：
    CSB-EP309724DLU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    黑腹果蝇smo重组蛋白
  • 货号：
    CSB-EP309724DLU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    黑腹果蝇smo重组蛋白
  • 货号：
    CSB-BP309724DLU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    黑腹果蝇smo重组蛋白
  • 货号：
    CSB-MP309724DLU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    smo
  • Uniprot No.：
    P91682
  • 别名：
    smo; CG11561; Protein smoothened; SMOH; Smooth; dSMO
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Segment polarity protein required for correct patterning of every segment. G protein-coupled receptor that associates with the patched protein (ptc) to transduce the hedgehog (hh) signal through the activation of an inhibitory G-protein. In the absence of hh, ptc represses the constitutive signaling activity of smo through fused (fu). Essential component of a hh-signaling pathway which regulates the Duox-dependent gut immune response to bacterial uracil; required to activate Cad99C-dependent endosome formation, norpA-dependent Ca2+ mobilization and p38 MAPK, which are essential steps in the Duox-dependent production of reactive oxygen species (ROS) in response to intestinal bacterial infection.
  • 基因功能参考文献：
    1. A novel mechanism for Smo activation by sumoylation that is regulated by Hh and Smo interacting proteins is uncovered. PMID: 28195188
    2. Data show that Hedgehog (Hh) stimulates the binding of Smoothened (Smo) to a plasma membrane-associated kinase Gilgamesh (Gish)/CK1gamma and that Gish fine-tunes Hh pathway activity by phosphorylating a Ser/Thr cluster of Smo carboxyl-terminal intracellular tail (C-tail). PMID: 27280464
    3. sumoylation acts in parallel with phosphorylation to promote Smo cell-surface expression and Hh signaling. PMID: 27746045
    4. PI(4)P directly binds Smo through an arginine motif, which then triggers Smo phosphorylation and activation. PMID: 26863604
    5. Smo protein can bias its signal and post-translational modification can impact this process. PMID: 26291458
    6. Hedgehog-regulated atypical PKC promotes phosphorylation and activation of Smoothened and Cubitus interruptus in Drosophila. PMID: 25349414
    7. Gprk2 phosphorylation in the membrane proximal C-terminus is an evolutionarily ancient mechanism of Smo regulation, and point to a higher degree of similarity in the regulation and signaling mechanisms of bilaterian Smo proteins PMID: 25009998
    8. Hh induced a switch from the association of PKAc with a cytosolic complex of Ci and the kinesin-like protein Costal2 (Cos2) to a membrane-bound Smo-Cos2 complex. PMID: 24985345
    9. findings demonstrate that deregulated Hedgehog signaling driven by active Smoothened mutants is attenuated by ER stressors that induce unfolded protein response (UPR); upon UPR induction, active Smoothened mutants are targeted by ER-associated degradation, resulting in attenuation of inappropriate pathway activity PMID: 23572559
    10. Hh gradient signals, Smo transduces high level Hh signal by forming oligomers/higher order clusters in the lipid rafts of cell plasma membrane. PMID: 23532857
    11. the differential phosphorylation of Smo mediates the thresholds of Hh activity PMID: 22537496
    12. Results indicate that miR-5 can specifically suppress hedgehog (Hh) signaling by directly targeting smoothened (Smo) in Drosophila. PMID: 23085394
    13. Hh-induced phosphorylation promotes Smo cell surface accumulation by inhibiting its ubiquitination, which contributes to Hh pathway activation. PMID: 22253574
    14. These findings are consistent with loop cysteines engaging in disulfide bonds that facilitate a Smoothened conformation that is silent in the absence of Hedgehog, but can transition to a fully active state in response to ligand. PMID: 22223683
    15. established a functional link between sequentially phosphorylated Smo species and graded Hh activity PMID: 21730325
    16. Characterization of metabolic phenotypes in the medulloblastoma of smo transgenic mice. PMID: 20842126
    17. Gprk2 promotes Smo activation by phosphorylating Smo C-terminal tail (C-tail) at Ser741/Thr742, which is facilitated by PKA and CK1 phosphorylation at adjacent Ser residues PMID: 20844016
    18. results suggest a model in which Ptc directly or indirectly functions to suppress the accumulation of PI4P. Binding of Hh to Ptc derepresses the levels of PI4P, which, in turn, promotes Smo activation. PMID: 20643350
    19. Patched normally regulates Smo degradation by changing the lipid composition of endosomes PMID: 19906846
    20. These results provide insight into head morphogenesis and reveal an unexpected positive role for Ptc-Smo signaling in promoting cell proliferation. PMID: 11934850
    21. Smo transduces the Hh signal by physically interacting with the Cos2/Fu protein complex. PMID: 14597665
    22. Data show that a positive response of Costal2 to Hedgehog stimulation requires a portion of the Smoothened cytoplasmic tail and the Costal2 cargo domain, which interacts directly with Smoothened. PMID: 14636583
    23. Smo mediates both inhibition of the endosome-associated Hedgehog signaling complex-repressor and activation of Hedgehog signaling complex-activated at the plasma membrane in response to Hh and through interaction with Cos2 PMID: 14645371
    24. Smo protein requires the amino terminus and the juxtamembrane region of the carboxy terminus for normal activity and accumulates in the plasma membrane PMID: 15172682
    25. Smoothened, similarly distributed to those regulating Ci, are essential for Smoothened to transduce a hedgehog signal and for normal regulation of Smoothened protein levels. PMID: 15592457
    26. Smoothened is phosphorylated in Hedgehog pathway activation PMID: 15598741
    27. Hh induces progressive Smo phosphorylation by PKA and CKI, leading to elevation of Smo cell-surface levels and signalling activity. PMID: 15616566
    28. sm belongs to a family of heterogeneous nuclear ribonucleoprotein (hnRNP; it is proposed to control axonal navigation and connectivity which is partly achieved at the level of mRNA splicing or exporting. PMID: 15676023
    29. Smoothened regulates activator and repressor functions of Hedgehog signaling via two distinct mechanisms PMID: 16423832
    30. There is evidence of differences between Drosophila and vertebrates concerning signalling downstream of Smo, as well as in Smo itself. PMID: 16516476
    31. Map kinase cytoplasmic hold is genetically dependent on Hedgehog and Decapentaplegic (Dpp) receptors Smo and Tkv. PMID: 16540506
    32. the upstream part of the Hedgehog pathway involving Hedgehog interaction with Patched, regulation of Smoothened by Patched, and Smoothened enrichment at the plasma membrane is highly conserved between Drosophila and humans PMID: 16867986
    33. The last 59 amino acids of Smoothened cytoplasmic tail directly bind the protein kinase Fused and negatively regulate the Hedgehog pathway. PMID: 17182028
    34. Results show that Smoothened, Sex-lethal, and Patched co-immunoprecipitate and co-fractionate, suggesting a large complex of both membrane and cytoplasmic components of the Hedgehog pathway. PMID: 17284519
    35. Hedgehog induced phosphorylation activates SMO by inducing a conformational switch PMID: 17960137
    36. results demonstrate that Smo functions as a canonical GPCR, which signals through Galphai to regulate Hh pathway activation PMID: 18987629
    37. Hh downregulates Smo-PP4 interaction that is mediated by Cos2. PMID: 19088085

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  • 亚细胞定位：
    Cell membrane; Multi-pass membrane protein.
  • 蛋白家族：
    G-protein coupled receptor Fz/Smo family
  • 数据库链接：

    KEGG: dme:Dmel_CG11561

    STRING: 7227.FBpp0077788

    UniGene: Dm.244

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