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  4. Recombinant Drosophila melanogaster Protein male-specific lethal-2 (msl-2), partial

Recombinant Drosophila melanogaster Protein male-specific lethal-2 (msl-2), partial

  • 中文名称：
    黑腹果蝇msl-2重组蛋白
  • 货号：
    CSB-YP343865DLU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    黑腹果蝇msl-2重组蛋白
  • 货号：
    CSB-EP343865DLU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    黑腹果蝇msl-2重组蛋白
  • 货号：
    CSB-EP343865DLU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    黑腹果蝇msl-2重组蛋白
  • 货号：
    CSB-BP343865DLU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    黑腹果蝇msl-2重组蛋白
  • 货号：
    CSB-MP343865DLU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    msl-2
  • Uniprot No.：
    P50534
  • 别名：
    msl-2; CG3241; E3 ubiquitin-protein ligase msl-2; EC 2.3.2.-; E3 ubiquitin-protein transferase msl-2; Protein male-specific lethal-2
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The Msl proteins are essential for elevating transcription of the single X chromosome in the male (X chromosome dosage compensation). Msl-2 is required for translation and/or stability of msl-1 in males. In complex with msl-1, acts as an E3 ubiquitin ligase that promotes ubiquitination of histone H2B.
  • 基因功能参考文献：
    1. the only male-specific protein within the Male-Specific Lethal complex; in addition to regulating the X chromosome, targets autosomal genes involved in patterning and morphogenesis PMID: 30194291
    2. HOW is required for SXL to retain msl2 transcripts in the nucleus PMID: 23788626
    3. Solution structure of MSL2 CXC domain reveals an unusual Zn3Cys9 cluster and similarity to pre-SET domains of histone lysine methyltransferases. PMID: 23029009
    4. MSL2 can fulfill its role as the central regulator of the faithful biogenesis and functionality of the dosage compensation(DC) machinery. PMID: 23084834
    5. A model is proposed in which a non-chromatin-associated partial or complete MSL-complex titrates newly transcribed msl2 mRNA and thus regulates the amount of available MSL complex by feedback. PMID: 21551218
    6. Reporter gene assays and localization of GFP-fusion proteins confirm the important contribution of the CXC domain of MSL2 for Dosage Compensation Complex targeting in vivo. PMID: 20139418
    7. suggest a model in which male-specific lethal (MSL) proteins assemble into active complexes by binding nascent roX transcripts PMID: 12446910
    8. spreads from roX genes on the Drosophila X chromosome. PMID: 12782651
    9. dSLX binds to the 3'UTR of msl-2 mRNA and activates the translational repressor domain, thereby enabling it to recruit co-repressors in a specific fashion. PMID: 14532129
    10. MSL2 controls male-specific roX expression in the absence of any other MSL protein. PMID: 15126401
    11. Here, we uncover the mechanism by which SXL achieves tight control of msl-2 translation initiation. This failsafe mechanism thus forms the molecular basis of a critical step in dosage compensation. PMID: 16122421
    12. the association of MSL2 with the X chromosome is exceptionally stable, essentially excluding dynamic redistribution of the dosage compensation complex during interphase PMID: 16179989
    13. X chromosome binding of MSL1 requires a conserved leucine zipper-like motif that binds to MSL2. PMID: 16199870
    14. Our results reveal a novel functional role for UNR as a translational repressor and indicate that UNR is a key component of a "fail-safe" dosage compensation regulatory system that prevents toxic MSL-2 synthesis in female cells PMID: 16452508
    15. Results suggest that the MSL complex (including MSL-1, -2, and -3) uses high-affinity sites to initially recognize the X chromosome and then associates with many of its targets through sequence-independent features of transcribed genes. PMID: 17936709
    16. Incorporation of the roX RNAs into the MSL complex alters the binding specificity of the chromatin-binding module formed by the amino-terminal domains of MSL1 and MSL2. PMID: 18086881
    17. in metafemales with ectopically expressed MSL2, the autosomal expression is returned to a more normal level. PMID: 19556782
    18. Efficient msl-2 mRNA silencing via the 3' UTR requires both a poly(A) tail and PABP function, indicating that UNR directly interacts with PABP. PMID: 19941818

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  • 亚细胞定位：
    Nucleus. Chromosome. Note=Msl-1 and msl-2 colocalize to many sites on the male X chromosome.
  • 蛋白家族：
    MSL2 family
  • 数据库链接：

    KEGG: dme:Dmel_CG3241

    STRING: 7227.FBpp0077259

    UniGene: Dm.14311

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