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  4. Recombinant Drosophila melanogaster Protein-cysteine N-palmitoyltransferase porcupine (por), partial

Recombinant Drosophila melanogaster Protein-cysteine N-palmitoyltransferase porcupine (por), partial

  • 中文名称：
    黑腹果蝇por重组蛋白
  • 货号：
    CSB-YP895207DLU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    黑腹果蝇por重组蛋白
  • 货号：
    CSB-EP895207DLU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    黑腹果蝇por重组蛋白
  • 货号：
    CSB-EP895207DLU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    黑腹果蝇por重组蛋白
  • 货号：
    CSB-BP895207DLU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    黑腹果蝇por重组蛋白
  • 货号：
    CSB-MP895207DLU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    por
  • Uniprot No.：
    Q9VWV9
  • 别名：
    por; porc; CG6205; Protein-serine O-palmitoleoyltransferase porcupine; EC 2.3.1.250
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Protein-serine O-palmitoleoyltransferase that acts as a key regulator of the Wnt signaling pathway by mediating the attachment of palmitoleate, a 16-carbon monounsaturated fatty acid (C16:1), to Wnt proteins. Serine palmitoleylation of Wnt proteins is required for efficient binding to frizzled receptors. Also facilitates the glycosylation of Wnt family members, including wg and Wnt5. The cotranslational disulfide bond formation of wg competes with the N-glycosylation. Porc stimulates the post-translational N-glycosylation by anchoring wg at the ER membrane, probably through acylation.
  • 基因功能参考文献：
    1. Por-mediated lipidation of the S239-equivalent residue is essential for the interaction with, and secretion by, Wls. PMID: 22108505
    2. stimulates the posttranslational N-glycosylation of wingless in the endoplasmic reticulum PMID: 11821428
    3. WntD, a recently characterized Drosophila Wnt family member, does not require Porcupine or Wntless/Evi/Sprinter for its secretion or signaling activity PMID: 18430724
    4. Role of the porcupine gene in the development of the wing imaginal disk of Drosophila melanogaster PMID: 19137731
  • 亚细胞定位：
    Endoplasmic reticulum membrane; Multi-pass membrane protein.
  • 蛋白家族：
    Membrane-bound acyltransferase family, Porcupine subfamily
  • 数据库链接：

    KEGG: dme:Dmel_CG6205

    STRING: 7227.FBpp0305446

    UniGene: Dm.19165

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