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  4. Recombinant Drosophila melanogaster Protein PDF (Pdf)

Recombinant Drosophila melanogaster Protein PDF (Pdf)

  • 中文名称：
    黑腹果蝇Pdf重组蛋白
  • 货号：
    CSB-YP527273DLU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    黑腹果蝇Pdf重组蛋白
  • 货号：
    CSB-EP527273DLU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    黑腹果蝇Pdf重组蛋白
  • 货号：
    CSB-EP527273DLU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    黑腹果蝇Pdf重组蛋白
  • 货号：
    CSB-BP527273DLU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    黑腹果蝇Pdf重组蛋白
  • 货号：
    CSB-MP527273DLU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Pdf
  • Uniprot No.：
    O96690
  • 别名：
    Pdf; CG6496; Protein PDF [Cleaved into: PDF precursor-related peptide; PAP); Neuropeptide PDF; Pigment-dispersing factor homolog)]
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    Cytoplasmic domain
  • 表达区域：
    25-79
  • 氨基酸序列
    MAMPDE ERYVRKEYNR DLLDWFNNVG VGQFSPGQVA TLCRYPLILE NSLGPSVPI
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Neuropeptide PDF is the main transmitter regulating circadian locomotor rhythms. Required to maintain behavioral rhythms under constant conditions by coordinating pacemaker interactions in the circadian system. Ectopic expression induces long periods, while its absence leads to short periods. Acts on small and large ventral lateral neurons to control sleep and regulates the state transition from sleep to wake.
  • 基因功能参考文献：
    1. PDF feedback is required for maintaining normal oscillation pattern in CLK-driven transcription. PMID: 27732848
    2. PDF has dual functions in clock resetting and pacemaker output. PMID: 24643294
    3. The PDF-mediated increase in cAMP might lengthen circadian period by directly enhancing PER stability PMID: 24707054
    4. a neuroendocrine pathway, defined by PDF, couples the CNS to the physiological output of peripheral clock cells that produce pheromones. disruption of pathway reduces male sex pheromones and results in sex-specific differences in mating behavior. PMID: 23849197
    5. Tip60 regulates PDF expression in the small ventrolateral neurons. PMID: 22982579
    6. age-associated reduction of PDF may cause attenuation of intercellular communication in the circadian neuronal network and of TIM cycling, which may result in the age-related rhythm decay PMID: 23223368
    7. Autoreceptor control of peptide/neurotransmitter corelease from PDF neurons determines allocation of circadian activity in drosophila. PMID: 22938867
    8. findings support a hypothesis that PDF signaling components within target cells are sequestered into "circadian signalosomes," whose compositions differ between E and M pacemaker cell types. PMID: 22679392
    9. Intestinal PDF acts at a distance on the renal system, where it regulates ureter contractions. PMID: 22778427
    10. activation of PDF pathway alone by constant light conditions leads to the complex locomotor rhythm through desynchronized oscillation among some of the clock neurons PMID: 21708159
    11. Circadian pacemaker neurons expressing the neuropeptide PDF are especially important for morning anticipation and free-running rhythms. PMID: 20362452
    12. These results reveal the neuroanatomical basis of PDF's various circadian functions. PMID: 20011537
    13. Blocking Shibire function via overexpression of a dominant negative mutant of Shibire in PDF neurons slowed down the behavioral rhythm. This effect was absent in PDF receptor null mutants, indicating interference with PDF receptor-mediated endocytosis. PMID: 19916833
    14. The lateral neurons evening oscillator integrates light inputs and PDF signaling to control Drosophila diurnal behavior, revealing a new clock-independent function for PDF. PMID: 19820704
    15. Pdf is not required to maintain circadian protein oscillations under constant conditions; however, it is required to coordinate the phase and amplitude of such rhythms among the diverse pacemakers. PMID: 15356209
    16. circadian rhythm in olfactory response persisted in the absence of the pigment-dispersing factor neuropeptide PMID: 15851530
    17. robust PDF cycling in sLNv termini plays no more than a minor role in the Drosophila circadian system and is apparently not even necessary for clock output function PMID: 16603675
    18. PDF signaling is not required for robust activity rhythms in constant light as opposed to its requirement in constant darkness. PMID: 18044989
    19. Cleavage by a member of the Drosophila neprilysin family of endopeptidases is the most likely mechanism for inactivating synaptic PDF and that neprilysin might have an important role in regulating PDF signals within circadian neural circuits. PMID: 18055635
    20. Data suggest that functionally relevant signals from pacemaker to non-pacemaker clock neurons and other downstream targets rely both on PDF signaling and pacemaker electrical activity. PMID: 18375861
    21. PDF has different effects on Drosophila's circadian clocks in the accessory medulla and in the dorsal brain PMID: 18838607
    22. significant difference in the regulatory mechanisms of Pdf transcription between Drosophila melanogaster and D. virilis PMID: 19153257

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  • 亚细胞定位：
    Secreted.
  • 蛋白家族：
    Arthropod PDH family
  • 组织特异性：
    Predominantly expressed in adult head. Expressed at higher level in males than in females. In adult brain, it is specifically expressed in the ventral lateral neurons (LNvs) as well as in 2-4 tritocerebral cells and 4-6 abdominal cells.
  • 数据库链接：

    KEGG: dme:Dmel_CG6496

    STRING: 7227.FBpp0084396

    UniGene: Dm.21687

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