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  4. Recombinant Drosophila melanogaster DNA topoisomerase 3-beta (Top3beta), partial

Recombinant Drosophila melanogaster DNA topoisomerase 3-beta (Top3beta), partial

  • 中文名称：
    黑腹果蝇Top3beta重组蛋白
  • 货号：
    CSB-YP530977DLU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    黑腹果蝇Top3beta重组蛋白
  • 货号：
    CSB-EP530977DLU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    黑腹果蝇Top3beta重组蛋白
  • 货号：
    CSB-EP530977DLU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    黑腹果蝇Top3beta重组蛋白
  • 货号：
    CSB-BP530977DLU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    黑腹果蝇Top3beta重组蛋白
  • 货号：
    CSB-MP530977DLU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Top3beta
  • Uniprot No.：
    O96651
  • 别名：
    Top3beta; TOP3; CG3458; DNA topoisomerase 3-beta; EC 5.6.2.1; DNA topoisomerase III beta
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. Weakly relaxes negative supercoils and displays a distinct preference for binding single-stranded DNA.
  • 基因功能参考文献：
    1. These results demonstrate that TDRD3 can enhance the biochemical activities of Top3beta on both DNA and RNA substrates, in addition to its function of targeting Top3beta to critical sites in subcellular compartments. PMID: 27582462
    2. The results of this study suggested that Top3beta acts as an RNA topoisomerase and works with FMRP to promote the expression of mRNAs that are crucial for synapse formation. PMID: 23912945
    3. studies suggest that the topo III enzyme behaves as a structure-specific endonuclease in vivo, providing a reversible DNA cleavage activity that is specific for unpaired regions in the DNA PMID: 12048241
  • 蛋白家族：
    Type IA topoisomerase family
  • 数据库链接：

    KEGG: dme:Dmel_CG3458

    STRING: 7227.FBpp0070891

    UniGene: Dm.2946

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