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  4. Recombinant Drosophila melanogaster Bipolar kinesin KRP-130 (Klp61F), partial

Recombinant Drosophila melanogaster Bipolar kinesin KRP-130 (Klp61F), partial

  • 中文名称：
    黑腹果蝇Klp61F重组蛋白
  • 货号：
    CSB-YP342037DLU
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    黑腹果蝇Klp61F重组蛋白
  • 货号：
    CSB-EP342037DLU
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    黑腹果蝇Klp61F重组蛋白
  • 货号：
    CSB-EP342037DLU-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    黑腹果蝇Klp61F重组蛋白
  • 货号：
    CSB-BP342037DLU
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    黑腹果蝇Klp61F重组蛋白
  • 货号：
    CSB-MP342037DLU
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    Klp61F
  • Uniprot No.：
    P46863
  • 别名：
    Klp61F; KLP2; CG9191; Kinesin-like protein Klp61F; Bipolar kinesin KRP-130
  • 种属：
    Drosophila melanogaster (Fruit fly)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Important role in mitotic dividing cells. Microtubule motor required for spindle body separation. Slow plus-end directed microtubule motor capable of cross-linking and sliding apart antiparallel microtubules, thereby pushing apart the associated spindle poles during spindle assembly and function. Forms cross-links between microtubules within interpolar microtubule bundles. Contributes to the length of the metaphase spindle, maintains the prometaphase spindle by antagonizing Ncd, drives anaphase B, and also contributes to normal chromosome congression, kinetochore spacing, and anaphase A rates. Displays microtubule-stimulated ATPase activity. Required for normal fusome organization. Required in non-mitotic cells for transport of secretory proteins from the Golgi complex to the cell surface.
  • 基因功能参考文献：
    1. KLP61F is required for spindle and centromere symmetry in oocytes. PMID: 27932541
    2. Kinesin-5 pauses at microtubule plus ends and enhances polymerization by stabilizing longitudinal tubulin-tubulin interactions. These activities underlie the ability of kinesin-5 to slide and stabilize microtubule bundles in cells. PMID: 26437877
    3. Klp61F knockdown inhibits post-Golgi transport in Drosophila S2 cells. PMID: 23857769
    4. A staggered antiparallel coiled-coil 'BASS' (bipolar assembly) domain directs the assembly of four kinesin-5 polypeptides into bipolar minifilaments. PMID: 23299893
    5. Tyrosines in the kinesin-5 (KLP61F)head domain are necessary for phosphorylation by Wee1 and for mitotic spindle integrity. PMID: 19800237
    6. KLP61F prevents Ncd-mediated collapse of a nonmicrotubule matrix derived from the interphase nucleus PMID: 15367580
    7. Kinesin-5 motors can function by cross-linking and sliding adjacent spindle microtubules without the need for a static spindle matrix. PMID: 18678711
    8. results suggest that during morphogenesis of the embryo mitotic spindle, KLP61F's crosslinking & sliding activities could facilitate accumulation of KLP61F within antiparallel interpolar microtubules at the equator PMID: 19062285
    9. KLP61F activity contributes to normal chromosome congression, kinetochore spacing, and anaphase A rates PMID: 19158379
    10. calculated the structure of a K5 motor bound to MTs at 9 A resolution, providing insight into this important interaction. PMID: 19285086
    11. The work tends to support the "sliding filament" mechanism for KLP61F action in Drosophila embryo spindles. PMID: 19291760

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  • 亚细胞定位：
    Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Cytoplasm.
  • 蛋白家族：
    TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family, BimC subfamily
  • 数据库链接：

    KEGG: dme:Dmel_CG9191

    STRING: 7227.FBpp0072616

    UniGene: Dm.804

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