Recombinant Dog Gastric triacylglycerol lipase (LIPF)

中文名称：

Recombinant Dog Gastric triacylglycerol lipase (LIPF)
货号：

CSB-MP012976DO
规格：

￥2376
图片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

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纯度：

Greater than 95% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

LIPF
Uniprot No.：

P80035
别名：

GL; Gastric lipase
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
蛋白长度：

Full Length of Mature Protein
来源：

Mammalian cell
分子量：

46.6 kDa
表达区域：

20-398aa
氨基酸序列

LFGKLHPTNPEVTMNISQMITYWGYPAEEYEVVTEDGYILGIDRIPYGRKNSENIGRRPVAFLQHGLLASATNWISNLPNNSLAFILADAGYDVWLGNSRGNTWARRNLYYSPDSVEFWAFSFDEMAKYDLPATIDFILKKTGQDKLHYVGHSQGTTIGFIAFSTNPKLAKRIKTFYALAPVATVKYTETLLNKLMLVPSFLFKLIFGNKIFYPHHFFDQFLATEVCSRETVDLLCSNALFIICGFDTMNLNMSRLDVYLSHNPAGTSVQNVLHWSQAVKSGKFQAFDWGSPVQNMMHYHQSMPPYYNLTDMHVPIAVWNGGNDLLADPHDVDLLLSKLPNLIYHRKIPPYNHLDFIWAMDAPQAVYNEIVSMMGTDNK
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
蛋白标签：

C-terminal 10xHis-tagged
产品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
缓冲液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
复溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
储存条件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保质期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
货期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

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功能：

Catalyzes the hydrolysis of triacylglycerols to yield free fatty acids, diacylglycerol, monoacylglycerol, and glycerol. Shows a preferential hydrolysis at the sn-3 position of triacylglycerol.
基因功能参考文献：
1. We used b-lactoglobulin and thermosensitive methylated nanocrystalline cellulose (metNCC) as model biopolymers to investigate the role of interfacial fluid dynamics and morphology for interfacial displacement processes by rDGL and polysorbate 20 (P20) under gastric conditions. PMID: 27635994
2. insights into the domain movements PMID: 20965171
3. results show that gastric lipase also acts in solution on vinyl butyrate, with an optimum activity above pH 7, which suggests that gastric lipase is able to hydrolyze ester bonds via the classical mechanism of serine hydrolases PMID: 16411775
4. Investigation of the influence of feeding and fasting on the daily rhythm of serum lipase activity in dogs. PMID: 18987232
亚细胞定位：

Secreted.
蛋白家族：

AB hydrolase superfamily, Lipase family
组织特异性：

Expressed in the mucous pit cells of gastric glands (at protein level). Expressed in the fundic and pyloric mucosa.
数据库链接：

KEGG: cfa:403867

STRING: 9615.ENSCAFP00000023039

UniGene: Cfa.3733

Recombinant Dog Gastric triacylglycerol lipase (LIPF)

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