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  4. Recombinant Dictyostelium discoideum Myosin-2 heavy chain (mhcA), partial

Recombinant Dictyostelium discoideum Myosin-2 heavy chain (mhcA), partial

  • 中文名称：
    盘基网柄菌mhcA重组蛋白
  • 货号：
    CSB-YP357489DKK
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    盘基网柄菌mhcA重组蛋白
  • 货号：
    CSB-EP357489DKK
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    盘基网柄菌mhcA重组蛋白
  • 货号：
    CSB-EP357489DKK-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    盘基网柄菌mhcA重组蛋白
  • 货号：
    CSB-BP357489DKK
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    盘基网柄菌mhcA重组蛋白
  • 货号：
    CSB-MP357489DKK
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    mhcA
  • Uniprot No.：
    P08799
  • 别名：
    mhcA; DDB_G0286355; Myosin-2 heavy chain; Myosin II heavy chain
  • 种属：
    Dictyostelium discoideum (Slime mold)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Myosin is a protein that binds to actin and has ATPase activity that is activated by actin.
  • 基因功能参考文献：
    1. Regulation and plasticity of catalysis in myosin II are reported based on insights from the analysis of mechanochemical coupling in this protein. PMID: 28225609
    2. Switch 1 loop movement in myosin II determines actin interaction. PMID: 17213877
    3. a structural transition in the Dictyostelium myosin II relay helix during the actin-activated power stroke, is reported. PMID: 23589853
    4. Myosin heads bound to oriented actin filaments are highly disordered with respect to the actin filament axis, in contrast to the nearly crystalline order of myosin heads in rigor. PMID: 23250750
    5. Oxidation-induced structural change in myosin includes a redistribution of existing structural states of the actin-binding cleft. PMID: 21988699
    6. analysis of the structural basis for the allosteric interference of myosin function by reactive thiol region mutations G680A and G680V PMID: 21841195
    7. These results demonstrate that the PP2A B subunits phr2aBalpha and phr2aBbeta contribute to myosin II assembly control in vivo, with phr2aBalpha having the predominant role facilitating MHC dephosphorylation to facilitate filament assembly. PMID: 21357476

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  • 亚细胞定位：
    Cytoplasm, cell cortex. Note=Highest concentration in the posterior cell cortex.
  • 蛋白家族：
    TRAFAC class myosin-kinesin ATPase superfamily, Myosin family
  • 数据库链接：

    KEGG: ddi:DDB_G0286355

    STRING: 44689.DDB0191444

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