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  4. Recombinant Danio rerio V (D)J recombination-activating protein 1, partial

Recombinant Danio rerio V (D)J recombination-activating protein 1, partial

  • 中文名称：
    斑马鱼rag1重组蛋白
  • 货号：
    CSB-YP517518DIL
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    斑马鱼rag1重组蛋白
  • 货号：
    CSB-EP517518DIL
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    斑马鱼rag1重组蛋白
  • 货号：
    CSB-EP517518DIL-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    斑马鱼rag1重组蛋白
  • 货号：
    CSB-BP517518DIL
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    斑马鱼rag1重组蛋白
  • 货号：
    CSB-MP517518DIL
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    rag1
  • Uniprot No.：
    O13033
  • 别名：
    rag1; V(D)J recombination-activating protein 1; RAG-1) [Includes: Endonuclease RAG1; EC 3.1.-.-); E3 ubiquitin-protein ligase RAG1; EC 2.3.2.27; RING-type E3 ubiquitin transferase RAG1)]
  • 种属：
    Danio rerio (Zebrafish) (Brachydanio rerio)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Catalytic component of the RAG complex, a multiprotein complex that mediates the DNA cleavage phase during V(D)J recombination. V(D)J recombination assembles a diverse repertoire of immunoglobulin and T-cell receptor genes in developing B and T lymphocytes through rearrangement of different V (variable), in some cases D (diversity), and J (joining) gene segments. In the RAG complex, RAG1 mediates the DNA-binding to the conserved recombination signal sequences (RSS) and catalyzes the DNA cleavage activities by introducing a double-strand break between the RSS and the adjacent coding segment. RAG2 is not a catalytic component but is required for all known catalytic activities. DNA cleavage occurs in 2 steps: a first nick is introduced in the top strand immediately upstream of the heptamer, generating a 3'-hydroxyl group that can attack the phosphodiester bond on the opposite strand in a direct transesterification reaction, thereby creating 4 DNA ends: 2 hairpin coding ends and 2 blunt, 5'-phosphorylated ends. In addition to its endonuclease activity, RAG1 also acts as an E3 ubiquitin-protein ligase that mediates monoubiquitination of histone H3. Histone H3 monoubiquitination is required for the joining step of V(D)J recombination.
  • 基因功能参考文献：
    1. Differential gene expression following TLR stimulation in rag1-/- mutant zebrafish tissues and morphological descriptions of lymphocyte-like cell populations PMID: 28910320
    2. first report of any rag1(-/-) mutant vertebrate mounting a protective secondary immune response to a bacterial pathogen PMID: 22970222
    3. target-selected inactivation of the rag1 gene PMID: 12098699
    4. Rag1 has a role in developing zebrafish cortex-medullary regionalization PMID: 12203724
    5. by embryonic day 4, rag1 transcripts are seen in the pancreas PMID: 12370418
    6. Recombination signal sequences are recognized by rag1 protein. PMID: 15829313
    7. Rag1 expression is restricted to a subpopulation of zebrafish olfactory neurons projecting to the lateral olfactory bulb; Rag11 catalytic activity is not essential for axon targeting PMID: 16018818
    8. This report describes the leukocyte populations in a unique model, recombination activation gene 1-/- mutant zebrafish (rag1 mutants). PMID: 19192305

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    RAG1 family
  • 组织特异性：
    In the adult, predominantly expressed in the thymus with lower levels in the pronephros, mesonephros and ovary.
  • 数据库链接：

    KEGG: dre:30663

    STRING: 7955.ENSDARP00000120100

    UniGene: Dr.133607

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