Your Good Partner in Biology Research

中文简体

CSF2｜ CTLA4｜ DLL3｜ EGFR｜ IL4｜ IL4R｜ LAG3｜ MSTN｜ NECTIN4｜ NT5E｜ SEZ6｜ TM4SF1｜ TNFSF13｜ TPO

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Chicken Villin-1 (VIL1), partial

Recombinant Chicken Villin-1 (VIL1), partial

  • 中文名称：
    鸡VIL1重组蛋白
  • 货号：
    CSB-YP025855CH
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    鸡VIL1重组蛋白
  • 货号：
    CSB-EP025855CH
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    鸡VIL1重组蛋白
  • 货号：
    CSB-EP025855CH-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    鸡VIL1重组蛋白
  • 货号：
    CSB-BP025855CH
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    鸡VIL1重组蛋白
  • 货号：
    CSB-MP025855CH
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    VIL1
  • Uniprot No.：
    P02640
  • 别名：
    VIL1; VIL; Villin-1
  • 种属：
    Gallus gallus (Chicken)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Epithelial cell-specific Ca(2+)-regulated actin-modifying protein that modulates the reorganization of microvillar actin filaments. Plays a role in the actin nucleation, actin filament bundle assembly, actin filament capping and severing. Binds phosphatidylinositol 4,5-bisphosphate (PIP2) and lysophosphatidic acid (LPA); binds LPA with higher affinity than PIP2. Binding to LPA increases its phosphorylation by SRC and inhibits all actin-modifying activities. Binding to PIP2 inhibits actin-capping and -severing activities but enhances actin-bundling activity. Regulates the intestinal epithelial cell morphology, cell invasion, cell migration and apoptosis. Protects against apoptosis induced by dextran sodium sulfate (DSS) in the gastrointestinal epithelium. Appears to regulate cell death by maintaining mitochondrial integrity. Enhances hepatocyte growth factor (HGF)-induced epithelial cell motility, chemotaxis and wound repair. Its actin-bundling activity is inhibited by tropomyosin.
  • 基因功能参考文献：
    1. The thermostable 36-residue subdomain of the villin headpiece (HP36) is the smallest known cooperatively folding protein. The side chain methyl dynamics of villin HP36 has been measured across a wide range of temperatures. PMID: 26473993
    2. A compact native 24-residues-long supersecondary structure derived from the hyperstable villin headpiece subdomain consisting of helices 2 and 3, is described. PMID: 25650934
    3. side-chain dynamics of several key hydrophobic methyl groups in chicken villin headpiece subdomain (HP36) PMID: 22085262
    4. a buried salt bridge is essential for villin headpiece folding and actin binding PMID: 21449557
    5. detailed comparison of these crystal structures with each other and to our previously determined NMR structures of HP67 PMID: 16142894
    6. Results indicate that reduced conformational flexibility of the N-terminal subdomain in wild-type villin HP67 is due to intramolecular interactions with the His41 imidazole ring. PMID: 16332376
    7. Results presented here demonstrate that unfolding of the villin headpiece is a multistate process. PMID: 16337228
    8. a crucial role for this proline residue in structural stability and folding potential of HP (sub)domains consistent with Pro-Trp stacking as a more general determinant of protein stability PMID: 16697408
    9. Villin headpiece domain HP67 & its mutant (H41Y) are resolved in different crystal form & space groups. 2 molecules occur in each asymmetric unit in the new space group P6(1) differing in harmonic motions. PMID: 19390152

    显示更多

    收起更多

  • 亚细胞定位：
    Cytoplasm, cytoskeleton. Cell projection, microvillus. Cell projection, lamellipodium. Cell projection, ruffle. Cell projection, filopodium tip. Cell projection, filopodium.
  • 蛋白家族：
    Villin/gelsolin family
  • 组织特异性：
    Specifically expressed in epithelial cells. Component of brush border microvilli.
  • 数据库链接：

    KEGG: gga:396423

    STRING: 9031.ENSGALP00000037398

    UniGene: Gga.816

Most popular with customers

产品中心
特别关注


X
九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |