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  4. Recombinant Chicken Piwi-like protein 1 (PIWIL1), partial

Recombinant Chicken Piwi-like protein 1 (PIWIL1), partial

  • 中文名称：
    鸡PIWIL1重组蛋白
  • 货号：
    CSB-YP018045CH
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    鸡PIWIL1重组蛋白
  • 货号：
    CSB-EP018045CH
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    鸡PIWIL1重组蛋白
  • 货号：
    CSB-EP018045CH-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    鸡PIWIL1重组蛋白
  • 货号：
    CSB-BP018045CH
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    鸡PIWIL1重组蛋白
  • 货号：
    CSB-MP018045CH
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PIWIL1
  • Uniprot No.：
    A6N7Y9
  • 别名：
    PIWIL1; Piwi-like protein 1; EC 3.1.26.-
  • 种属：
    Gallus gallus (Chicken)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Endoribonuclease that plays a central role in postnatal germ cells by repressing transposable elements and preventing their mobilization, which is essential for the germline integrity. Acts via the piRNA metabolic process, which mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and govern the methylation and subsequent repression of transposons. Directly binds methylated piRNAs, a class of 24 to 30 nucleotide RNAs that are generated by a Dicer-independent mechanism and are primarily derived from transposons and other repeated sequence elements. Strongly prefers a uridine in the first position of their guide (g1U preference, also named 1U-bias). Besides their function in transposable elements repression, piRNAs are probably involved in other processes during meiosis such as translation regulation. Not involved in the piRNA amplification loop, also named ping-pong amplification cycle. Acts as an endoribonuclease that cleaves transposon messenger RNAs.
  • 基因功能参考文献：
    1. we show the negative correlation between promoter methylation and piwil1 expression and that the spatiotemporal expression of chicken Piwil1 from the PGC stage to the round spermatid stage is influenced by methylation-mediated transcription factor regulation. PMID: 27108736
    2. Piwil1 plays a key role in meiosis during chicken spermatogenesis. PMID: 26811260
    3. The screened SNPs can be used as candidate markers for Piwi, and our results provide basic information for the further study of Piwi function in poultry. PMID: 26600541
    4. expression during spermatogenesis regulated by DNA methylation and NF-Y PMID: 26471838
    5. The Piwi-like 1 (Piwil1) gene was cloned and its expression was characterized in the Langshan chickens. PMID: 24197694

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Argonaute family, Piwi subfamily
  • 数据库链接：

    KEGG: gga:416804

    STRING: 9031.ENSGALP00000004162

    UniGene: Gga.16962

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