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  4. Recombinant Bovine Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial (DBT)

Recombinant Bovine Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial (DBT)

  • 中文名称：
    Recombinant Bovine Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial(DBT)
  • 货号：
    CSB-YP006530BO
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Bovine Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial(DBT)
  • 货号：
    CSB-EP006530BO
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Bovine Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial(DBT)
  • 货号：
    CSB-EP006530BO-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Bovine Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial(DBT)
  • 货号：
    CSB-BP006530BO
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Bovine Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex, mitochondrial(DBT)
  • 货号：
    CSB-MP006530BO
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    DBT
  • Uniprot No.：
    P11181
  • 别名：
    DBT; Lipoamide acyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; mitochondrial; EC 2.3.1.168; Branched-chain alpha-keto acid dehydrogenase complex component E2; BCKAD-E2; BCKADE2; Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto acid dehydrogenase complex; Dihydrolipoamide branched chain transacylase; Dihydrolipoyllysine-residue; 2-methylpropanoyl)transferase
  • 种属：
    Bos taurus (Bovine)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    62-482
  • 氨基酸序列
    GQIVQFKLS DIGEGIREVT VKEWYVKEGD TVSQFDSICE VQSDKASVTI TSRYDGVIKK LYYNLDDTAY VGKPLVDIET EALKDSEEDV VETPAVSHDE HTHQEIKGQK TLATPAVRRL AMENNIKLSE VIGSGKDGRI LKEDILNYLE KQTGAILPPS PKAEIMPPPP KPKDRTIPIP ISKPPVFIGK DRTEPVKGFH KAMVKTMSAA LKIPHFGYCD EVDLTELVKL REELKPIAFA RGIKLSFMPF FLKAASLGLL QFPILNASVD ENCQNITYKA SHNIGIAMDT EQGLIVPNVK NVQIRSIFEI ATELNRLQKL GSAGQLSTND LIGGTFTLSN IGSIGGTYAK PVILPPEVAI GALGTIKALP RFNEKGEVCK AQIMNVSWSA DHRIIDGATV SRFSNLWKSY LENPAFMLLD LK
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of three enzymatic components: branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransferase (E2) and lipoamide dehydrogenase (E3). Within this complex, the catalytic function of this enzyme is to accept, and to transfer to coenzyme A, acyl groups that are generated by the branched-chain alpha-keto acid decarboxylase component.
  • 基因功能参考文献：
    1. This gating mechanism synchronizes the binding of the two substrates to the active-site channel, which serves as a feed-forward switch to coordinate the E2b-catalyzed acyltransfer reaction. PMID: 17124494
  • 亚细胞定位：
    Mitochondrion matrix.
  • 蛋白家族：
    2-oxoacid dehydrogenase family
  • 组织特异性：
    Expressed in kidney (at protein level).
  • 数据库链接：

    KEGG: bta:280759

    STRING: 9913.ENSBTAP00000008292

    UniGene: Bt.107201

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