Recombinant Bacillus subtilis Transcription-repair-coupling factor (mfd), partial

中文名称：

枯草芽孢杆菌mfd重组蛋白
货号：

CSB-YP336298BRJ
规格：
来源：

Yeast
其他：

中文名称：

枯草芽孢杆菌mfd重组蛋白
货号：

CSB-EP336298BRJ
规格：
来源：

E.coli
其他：

中文名称：

枯草芽孢杆菌mfd重组蛋白
货号：

CSB-EP336298BRJ-B
规格：
来源：

E.coli
共轭：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名称：

枯草芽孢杆菌mfd重组蛋白
货号：

CSB-BP336298BRJ
规格：
来源：

Baculovirus
其他：

中文名称：

枯草芽孢杆菌mfd重组蛋白
货号：

CSB-MP336298BRJ
规格：
来源：

Mammalian cell
其他：

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纯度：

>85% (SDS-PAGE)
基因名：

mfd
Uniprot No.：

P37474
别名：

mfd; BSU00550Transcription-repair-coupling factor; TRCF; EC 3.6.4.-
种属：

Bacillus subtilis (strain 168)
蛋白长度：

Partial
蛋白标签：

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
产品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
复溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
储存条件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保质期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
货期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

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功能：

Couples transcription and DNA repair by recognizing RNA polymerase (RNAP) stalled at DNA lesions. Mediates ATP-dependent release of RNAP and its truncated transcript from the DNA, and recruitment of nucleotide excision repair machinery to the damaged site.
基因功能参考文献：
1. Data propose that the Mfd-dependent repair pathway operates during B. subtilis sporulation and that its function is required to eliminate genetic damage from transcriptionally active genes. PMID: 24118570
2. The authors examined the role of Mfd, a transcription elongation factor involved in DNA repair, in this process and showed that proficiency of this factor promotes mutagenic events. PMID: 22248542
3. This idea is supported by the finding that the transcription-repair coupling factor Mfd is required for the decryptification of gudB. PMID: 22178973
4. Here we demonstrate that the loss of Mfd has a depressive effect on stationary-phase mutagenesis. PMID: 16950921
亚细胞定位：

Cytoplasm.
蛋白家族：

UvrB family; Helicase family, RecG subfamily
数据库链接：

KEGG: bsu:BSU00550

STRING: 224308.Bsubs1_010100000280

Recombinant Bacillus subtilis Transcription-repair-coupling factor (mfd), partial

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