Recombinant Bacillus subtilis Stage II sporulation protein E (spoIIE), partial

中文名称：

枯草芽孢杆菌spoIIE重组蛋白
货号：

CSB-YP327679BRJ
规格：
来源：

Yeast
其他：

中文名称：

枯草芽孢杆菌spoIIE重组蛋白
货号：

CSB-EP327679BRJ
规格：
来源：

E.coli
其他：

中文名称：

枯草芽孢杆菌spoIIE重组蛋白
货号：

CSB-EP327679BRJ-B
规格：
来源：

E.coli
共轭：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名称：

枯草芽孢杆菌spoIIE重组蛋白
货号：

CSB-BP327679BRJ
规格：
来源：

Baculovirus
其他：

中文名称：

枯草芽孢杆菌spoIIE重组蛋白
货号：

CSB-MP327679BRJ
规格：
来源：

Mammalian cell
其他：

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纯度：

>85% (SDS-PAGE)
基因名：

spoIIE
Uniprot No.：

P37475
别名：

spoIIE; spoIIH; BSU00640; Stage II sporulation protein E; EC 3.1.3.16; Stage II sporulation protein H
种属：

Bacillus subtilis (strain 168)
蛋白长度：

Partial
蛋白标签：

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
产品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
复溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
储存条件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保质期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
货期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

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功能：

Normally needed for pro-sigma E processing during sporulation but can be bypassed in vegetative cells. Activates SpoIIAA by dephosphorylation.
基因功能参考文献：
1. using super-resolution microscopy, we demonstrate that DivIVA and SpoIIE ultimately display a biased localization on the side of the polar septum that faces the smaller compartment in which sigmaF is activated PMID: 25101664
2. regions II and III play a critical role in polar septum formation and that specific amino acid substitutions in those regions affect the abilities of SpoIIE both to promote polar division and to localize to the division machinery PMID: 15866939
3. the bulk of the forespore chromosome is translocated naked into the forespore compartment. We propose that the translocation-stripping activity of SpoIIIE plays a key role in reprogramming developmental gene expression in the forespore. PMID: 18593879
亚细胞定位：

Cell membrane; Multi-pass membrane protein. Note=Polar septum.
数据库链接：

KEGG: bsu:BSU00640

STRING: 224308.Bsubs1_010100000325

Recombinant Bacillus subtilis Stage II sporulation protein E (spoIIE), partial

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