Recombinant Bacillus subtilis D-alanine--D-alanyl carrier protein ligase (dltA)

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中文名称：

枯草芽孢杆菌dltA重组蛋白
货号：

CSB-EP336594BRJ(A4)
规格：

￥2328
图片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP336594BRJ(A4) could indicate that this peptide derived from E.coli-expressed Bacillus subtilis (strain 168) dltA.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP336594BRJ(A4) could indicate that this peptide derived from E.coli-expressed Bacillus subtilis (strain 168) dltA.
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纯度：

Greater than 85% as determined by SDS-PAGE.
基因名：

dltA
Uniprot No.：

P39581
别名：

dltA; dae; BSU38500; ipa-5rD-alanine--D-alanyl carrier protein ligase; DCL; EC 6.2.1.54; D-alanine--poly(phosphoribitol) ligase subunit 1; D-alanine-activating enzyme; DAE
种属：

Bacillus subtilis (strain 168)
蛋白长度：

Full Length
来源：

E.coli
分子量：

59.8 kDa
表达区域：

1-503aa
氨基酸序列

MKLLHAIQTHAETYPQTDAFRSQGQSLTYQELWEQSDRAAAAIQKRISGEKKSPILVYGHMEPHMIVSFLGSVKAGHPYIPVDLSIPSERIAKIIESSGAELLIHAAGLSIDAVGQQIQTVSAEELLENEGGSVSQDQWVKEHETFYIIYTSGSTGNPKGVQISAANLQSFTDWICADFPVSGGKIFLNQAPFSFDLSVMDLYPCLQSGGTLHCVTKDAVNKPKVLFEELKKSGLNVWTSTPSFVQMCLMDPGFSQDLLPHADTFMFCGEVLPVSVAKALLERFPKAKIFNTYGPTEATVAVTSVEITNDVISRSESLPVGFAKPDMNIFIMDEEGQPLPEGEKGEIVIAGPSVSRGYLGEPELTEKAFFSHEGQWAYRTGDAGFIQDGQIFCQGRLDFQIKLHGYRMELEEIEFHVRQSQYVRSAVVIPYQPNGTVEYLIAAIVPEEHEFEKEFQLTSAIKKELAASLPAYMIPRKFIYQDHIQMTANGKIDRKRIGEEVLV
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
蛋白标签：

N-terminal 6xHis-tagged
产品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
缓冲液：

Tris-based buffer,50% glycerol
储存条件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保质期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
货期：

3-7 business days
注意事项：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

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功能：

Catalyzes the first step in the D-alanylation of lipoteichoic acid (LTA), the activation of D-alanine and its transfer onto the D-alanyl carrier protein (Dcp) DltC. In an ATP-dependent two-step reaction, forms a high energy D-alanyl-AMP intermediate, followed by transfer of the D-alanyl residue as a thiol ester to the phosphopantheinyl prosthetic group of the Dcp. D-alanylation of LTA plays an important role in modulating the properties of the cell wall in Gram-positive bacteria, influencing the net charge of the cell wall.
基因功能参考文献：
1. DltA synthesizes not only N-(d-alanyl)-l-cysteine (a dipeptide) but also various oligopeptides. PMID: 26586916
2. assayed the substrate selectivity of DltA with its substrate DltC PMID: 15955059
3. analysis of the crystal structure of DltA PMID: 18784082
亚细胞定位：

Cytoplasm.
蛋白家族：

ATP-dependent AMP-binding enzyme family, DltA subfamily
数据库链接：

KEGG: bsu:BSU38500

STRING: 224308.Bsubs1_010100020786

Recombinant Bacillus subtilis D-alanine--D-alanyl carrier protein ligase (dltA)

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