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  4. Recombinant Bacillus subtilis Chromosome partition protein Smc (smc), partial

Recombinant Bacillus subtilis Chromosome partition protein Smc (smc), partial

  • 中文名称：
    枯草芽孢杆菌smc重组蛋白
  • 货号：
    CSB-YP347277BRJ
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    枯草芽孢杆菌smc重组蛋白
  • 货号：
    CSB-EP347277BRJ
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    枯草芽孢杆菌smc重组蛋白
  • 货号：
    CSB-EP347277BRJ-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    枯草芽孢杆菌smc重组蛋白
  • 货号：
    CSB-BP347277BRJ
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    枯草芽孢杆菌smc重组蛋白
  • 货号：
    CSB-MP347277BRJ
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    smc
  • Uniprot No.：
    P51834
  • 别名：
    smc; ylqA; BSU15940; Chromosome partition protein Smc
  • 种属：
    Bacillus subtilis (strain 168)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Required for chromosome condensation and partitioning.
  • 基因功能参考文献：
    1. Sandwiching of ATP molecules between Smc heads requires them to substantially tilt and translate relative to each other, thereby opening up the Smc arms. This mechanochemical gating reaction regulates chromosome targeting and propose a mechanism for DNA translocation based on the merging of DNA loops upon closure of Smc arms. PMID: 28689660
    2. we show that rapid inactivation of SMC or its partner protein ScpB during fast growth leads to a failure to resolve newly replicated origins and a complete block to chromosome segregation. PMID: 24440393
    3. A mutation in SMC that affects ATPase activity and results in exaggerated DNA binding causes a strong segregation defect and affects the localization of the SMC complex, which localizes to many more sites in the cell than under normal conditions PMID: 23475963
    4. An Smc dimer is bridged by a single ScpAB to generate asymmetric tripartite rings analogous to eukaryotic SMC complexes. PMID: 23353789
    5. has a dual function, in chromosome supercoiling and in active segregation PMID: 16272394

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  • 亚细胞定位：
    Cytoplasm. Note=Probably associated with the nucleoid.
  • 蛋白家族：
    SMC family
  • 数据库链接：

    KEGG: bsu:BSU15940

    STRING: 224308.Bsubs1_010100008791

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