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  4. Recombinant Arabidopsis thaliana Transcription factor PIF3 (PIF3), partial

Recombinant Arabidopsis thaliana Transcription factor PIF3 (PIF3), partial

  • 中文名称：
    拟南芥PIF3重组蛋白
  • 货号：
    CSB-YP524969DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥PIF3重组蛋白
  • 货号：
    CSB-EP524969DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥PIF3重组蛋白
  • 货号：
    CSB-EP524969DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥PIF3重组蛋白
  • 货号：
    CSB-BP524969DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥PIF3重组蛋白
  • 货号：
    CSB-MP524969DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PIF3
  • Uniprot No.：
    O80536
  • 别名：
    PIF3; BHLH8; EN100; PAP3; At1g09530; F14J9.19; Transcription factor PIF3; Basic helix-loop-helix protein 8; AtbHLH8; bHLH 8; Phytochrome-associated protein 3; Phytochrome-interacting factor 3; Transcription factor EN 100; bHLH transcription factor bHLH008
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor acting in the phytochrome signaling pathway. Activates transcription by binding to the G box (5'-CACGTG-3'). Acts as a negative regulator of phytochrome B signaling. Represses chlorophyll biosynthesis and photosynthesis in the dark. Recruits the histone deacetylase HDA15 to the promoters of chlorophyll biosynthetic and photosynthetic genes. HDA15 represses their transcription by histone deacetylation.
  • 基因功能参考文献：
    1. PIF degradation by DELLAs is required to coordinate light and gibberellins signals. PMID: 27282989
    2. study uncovers an important role of PIF3 in Arabidopsis freezing tolerance by negatively regulating the expression of genes in the CBF pathway PMID: 28739888
    3. PIF3 and TOC1 display coincident cobinding to dawn-phased genes under diurnal short-day conditions. They interact and colocalize in the nucleus. TOC1 acts directly as a transcriptional repressor of PIF3, which itself acts intrinsically as a transcriptional activator so that PIF3 and TOC1 act antagonistically in regulating the expression of their cotarget genes. PMID: 27071129
    4. Under negative day-night temperature difference, lower auxin biosynthesis activity limits the signaling in this pathway, resulting in low activity of PIF3 and short hypocotyls. PMID: 25516603
    5. PIF3 phosphorylation induces, and is necessary for, recruitment of LRB [Light-Response Bric-a-Brack/Tramtrack/Broad (BTB)] E3 ubiquitin ligases to the PIF3-phyB complex. PMID: 24904166
    6. Data indicate that HDA15 and PIF3 cotarget to the genes involved in chlorophyll biosynthesis and photosynthesis in the dark and repress gene expression. PMID: 23548744
    7. These results showed an altered response to light in seedlings with an impaired PIF3/MIDA regulatory network. PMID: 22499182
    8. directly activated by ETHYLENE-INSENSITIVE 3 and indispensible for ethylene-induced hypocotyl elongation in light PMID: 22818915
    9. The PIF3 protein abundance oscillates under diurnal conditions as a result of a progressive decline in PIF3 protein degradation mediated by photoactivated phyB, and consequent accumulation of the bHLH factor during the dark period. PMID: 22409654
    10. PIF3 regulates genes misexpressed in the dark, named MIDA genes PMID: 22108407
    11. PIF3 acts transiently, and its major function is to mediate phytochrome-induced signaling during the developmental switch from skotomorphogenesis to photomorphogenesis and/or dark to light transitions PMID: 15155879
    12. PIF3 may function in early phytochrome signaling at the dark-to-light transition, not only during initial seedling deetiolation, but daily at dawn under diurnal light-dark cycles. PMID: 15505214
    13. Data suggest that PIF3 may represent the primary intermolecular signaling transaction of the activated photoreceptor, tagging the target protein for proteosomal degradation. PMID: 16885032
    14. Data provide evidence that the mechanism by which PIFs3operate on the phyB signaling pathway under prolonged red light is through maintaining low phyB protein levels, in an additive or synergistic manner. PMID: 18252845
    15. Data show that dephosphorylation of PIF3 is mediated by phytochrome-associated protein phosphatase type 2C. PMID: 18564962
    16. PIF3 was circadian regulated in dark-grown seedlings. PMID: 19380736

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  • 亚细胞定位：
    Nucleus.
  • 数据库链接：

    KEGG: ath:AT1G09530

    STRING: 3702.AT1G09530.1

    UniGene: At.10926

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