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  4. Recombinant Arabidopsis thaliana Squamosa promoter-binding-like protein 7 (SPL7), partial

Recombinant Arabidopsis thaliana Squamosa promoter-binding-like protein 7 (SPL7), partial

  • 中文名称：
    拟南芥SPL7重组蛋白
  • 货号：
    CSB-YP837320DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥SPL7重组蛋白
  • 货号：
    CSB-EP837320DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥SPL7重组蛋白
  • 货号：
    CSB-EP837320DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥SPL7重组蛋白
  • 货号：
    CSB-BP837320DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥SPL7重组蛋白
  • 货号：
    CSB-MP837320DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SPL7
  • Uniprot No.：
    Q8S9G8
  • 别名：
    SPL7; At5g18830; F17K4.80; Squamosa promoter-binding-like protein 7
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Transcription factor that participates in reprogramming global gene expression during copper deficiency in order to improve the metal uptake and prioritize its distribution to copper proteins of major importance (Probable). Binds directly to 5'-GTAC-3' motifs in the microRNA (miRNA) promoter of the stress-responsive miRNAs miR398b and miR398c to activate their transcription. During copper deficiency, activates the copper transporters COPT1 and COPT2, and the copper chaperone CCH, directly or indirectly via miRNAs. Required for the expression of the miRNAs miR397, miR408 and miR857. Acts coordinately with HY5 to regulate miR408 and its target genes in response to changes in light and copper conditions. Activates miR857 and its target genes in response to low copper conditions. Involved in cadmium stress response by regulating miR397a, miR398b, miR398c and miR857. Required for iron homeostasis during copper deficiency.
  • 基因功能参考文献：
    1. Cytological and biochemical approaches confirmed an operative transmembrane domain (TMD) and uncovered a dual localisation of SPL7 between the nucleus and an endomembrane system, most likely the endoplasmic reticulum (ER). PMID: 25207797
    2. The data presented here provide evidence for SPL7 and KIN17 protein interaction as a point of convergence in response to both Copper deficiency and oxidative stress. PMID: 24335506
    3. SPL7 directly regulates miR408 expression. PMID: 23289771
    4. COPT6 expression is regulated by copper availability that, in part, is controlled by a master regulator of copper homeostasis, SPL7 PMID: 22865877
    5. Cu uptake into roots of Cu-deficient plants is dependent on FRO4/FRO5 and SPL7. PMID: 22374396
    6. SPL7 activates the transcription of multiple genes involved in copper homeostasis. PMID: 19122104

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  • 亚细胞定位：
    Nucleus speckle.
  • 组织特异性：
    Expressed in roots rosette leaves, cauline leaves, stems, flowers and siliques.
  • 数据库链接：

    KEGG: ath:AT5G18830

    STRING: 3702.AT5G18830.3

    UniGene: At.495

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