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  4. Recombinant Arabidopsis thaliana Sodium/hydrogen exchanger 1 (NHX1), partial

Recombinant Arabidopsis thaliana Sodium/hydrogen exchanger 1 (NHX1), partial

  • 中文名称：
    拟南芥NHX1重组蛋白
  • 货号：
    CSB-YP731586DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥NHX1重组蛋白
  • 货号：
    CSB-EP731586DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥NHX1重组蛋白
  • 货号：
    CSB-EP731586DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥NHX1重组蛋白
  • 货号：
    CSB-BP731586DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥NHX1重组蛋白
  • 货号：
    CSB-MP731586DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    NHX1
  • Uniprot No.：
    Q68KI4
  • 别名：
    NHX1; At5g27150; T21B4.60; Sodium/hydrogen exchanger 1; Na(+)/H(+) exchanger 1; NHE-1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Acts in low affinity electroneutral exchange of protons for cations such as Na(+) or K(+) across membranes. Can also exchange Li(+) and Cs(+) with a lower affinity. Involved in vacuolar ion compartmentalization necessary for cell volume regulation and cytoplasmic Na(+) detoxification. Required during leaves expansion, probably to stimulate epidermal cell expansion. Confers competence to grow in high salinity conditions.
  • 基因功能参考文献：
    1. Overexpression of AtNHX1 gene not only improved salt tolerance but also drought tolerance in transgenic groundnut. PMID: 21455815
    2. Studies shoe that the rutin content of the roots, stems and leaves of AtNHX1 transgenic buckwheat increased than those of the control plants. PMID: 17366888
    3. Use of the T-DNA insertional mutant of AtNHX1 (nhx1 plants) and DNA arrays to assess differences in transcriptional profiles and further characterize the roles of this protein. PMID: 15546358
    4. the presence of a vacuolar calmodulin-like protein acting on the vacuolar-localized AtNHX1 C terminus in a Ca(2+)- pH-dependent manner suggests the presence of signaling entities acting within the vacuole. PMID: 16249341
    5. AtNHX1 has a role in calcium signaling, sulfur metabolism, cell structure and cell growth, as well as vesicular trafficking and protein processing. PMID: 17411438
    6. To generate salt-tolerant turf and forage, tall fescue (Festuca arundinacea) was transformed with AtNHX1. PMID: 17561307
    7. Poisoning by toxic cations in the endosome/prevacuolar compartment is detrimental for cell functions, but it can be alleviated by improving the discrimination of transported alkali cations by the resident cation/H(+) antiporter. PMID: 19307188

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  • 亚细胞定位：
    Vacuole membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus membrane; Multi-pass membrane protein. Note=Tonoplast. Possibly also present in endoplasmic reticulum (ER) and Golgi apparatus.
  • 蛋白家族：
    Monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family
  • 组织特异性：
    Ubiquitous, with higher levels around vascular tissues and guard cells.
  • 数据库链接：

    KEGG: ath:AT5G27150

    STRING: 3702.AT5G27150.1

    UniGene: At.25435

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