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  4. Recombinant Arabidopsis thaliana Serine/threonine-protein kinase SRK2E (SRK2E)

Recombinant Arabidopsis thaliana Serine/threonine-protein kinase SRK2E (SRK2E)

  • 中文名称：
    拟南芥SRK2E重组蛋白
  • 货号：
    CSB-YP835970DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥SRK2E重组蛋白
  • 货号：
    CSB-EP835970DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥SRK2E重组蛋白
  • 货号：
    CSB-EP835970DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥SRK2E重组蛋白
  • 货号：
    CSB-BP835970DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥SRK2E重组蛋白
  • 货号：
    CSB-MP835970DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    SRK2E
  • Uniprot No.：
    Q940H6
  • 别名：
    SRK2E; OST1; SNRK2.6; At4g33950; F17I5.140; Serine/threonine-protein kinase SRK2E; EC 2.7.11.1; Protein OPEN STOMATA 1; SNF1-related kinase 2.6; SnRK2.6; Serine/threonine-protein kinase OST1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    full length protein
  • 表达区域：
    1-362
  • 氨基酸序列
    MDRPAVSGPM DLPIMHDSDR YELVKDIGSG NFGVARLMRD KQSNELVAVK YIERGEKIDE NVKREIINHR SLRHPNIVRF KEVILTPTHL AIVMEYASGG ELFERICNAG RFSEDEARFF FQQLISGVSY CHAMQVCHRD LKLENTLLDG SPAPRLKICD FGYSKSSVLH SQPKSTVGTP AYIAPEVLLK KEYDGKVADV WSCGVTLYVM LVGAYPFEDP EEPKNFRKTI HRILNVQYAI PDYVHISPEC RHLISRIFVA DPAKRISIPE IRNHEWFLKN LPADLMNDNT MTTQFDESDQ PGQSIEEIMQ IIAEATVPPA GTQNLNHYLT GSLDIDDDME EDLESDLDDL DIDSSGEIVY AM
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Activator of the abscisic acid (ABA) signaling pathway that regulates numerous ABA responses, such as stomata closure in response to drought, darkness, high CO(2), plant pathogens, or decreases in atmospheric relative humidity (RH). Involved in the resistance to drought by avoiding water loss. Required for the stomata closure mediated by pathogen-associated molecular pattern (PAMPs) (e.g. flg22 and LPS) of pathogenic bacteria such as P.syringae pv. tomato (Pst) and E.coli O157:H7. As a plant defense process, stomata are closed transiently in order to limit invaders, but actively reopened by bacteria after a few hours; virulent strains (e.g. Pst DC3000) are more efficient than avirulent strains (e.g. Pst DC3000 AvrRpt2) in reopening stomata. Mediates the phosphorylation and activation of the S-type anion efflux channel SLAC1, and thus promotes stomata closure. Essential for stomatal closure in response to reactive oxygen species (ROS). Promotes MAPKKK18 activity upon abscisic acid (ABA) treatment.
  • 基因功能参考文献：
    1. SnRK2.6 phosphorylates AKS1 in guard cells. PMID: 28438792
    2. OST1 was essential for methyl jasmonate (MeJA)-induced stomatal closure, while PYR1, PYL1, PYL2 and PYL4 ABA receptors were not sufficient factors. PMID: 27354421
    3. BAK1 regulates ABA-induced stomatal closure in guard cells PMID: 26724418
    4. CHYR1 interacted with SNF1-RELATED PROTEIN KINASE2 (SnRK2) kinases and could be phosphorylated by SnRK2.6 on the Thr-178 residue. PMID: 26508764
    5. OST1 functions downstream of ABAR in abscisic acid receptor signalling in guard cells. Consistent with this, ABAR protein is phosphorylated, but independently of the OST1 protein kinase. PMID: 26175350
    6. The role of OST1 in Yeast Elicitor-induced stomatal closure in Arabidopsis, was investigated. PMID: 25840086
    7. We have identified HAB1 C186 and C274 as H2O2-sensitive thiols and demonstrate that their oxidation inhibits both HAB1 catalytic activity and its ability to physically associate with SnRK2.6 by formation of intermolecular dimers PMID: 25460914
    8. SnRK2.6 is S-nitrosylated at cysteine 137, a residue adjacent to the kinase catalytic site. PMID: 25550508
    9. OST1 is a critical limiting component in abscissic acid regulation of stomatal apertures, ion channels and NADPH oxidases in Arabidopsis guard cells. PMID: 24033256
    10. OST1 is required for rapid stomatal closure in response to ozone, reduced air humidity and darkness induced stomatal regulation. PMID: 23703845
    11. The atabcg22 mutation further increased the water loss of srk2e/ost1 mutants, which were defective in ABA signalling in guard cells. PMID: 21575091
    12. ABI1 and OST1 represent regulatory elements for the abscissic acid-dependent activation of QUAC1-type guard cell anion currents. PMID: 23452338
    13. Results describe the cloning, expression, purification, crystallization and preliminary X-ray analysis of two mutants of OST1 from Arabidopsis thaliana. PMID: 21393844
    14. Data show that OST1 phosphorylates ABF3 on multiple LXRXXpS/T preferred motifs including T451 located in the midst of a conserved 14-3-3 binding site. PMID: 21085673
    15. Data show evidence for the involvement of distinct phosphorylation mechanisms in the activation of SnRK2.6 and SnRK2.10. PMID: 20561261
    16. OST1 and SLAC1 are regulators of the ROS-induced rapid stomatal closure. SLAC1 is phosphorylated by OST1. PMID: 20128877
    17. mediates hormonal and metabolic regulation of plant growth and developmen, implicated in the regulation of metabolic homeostasis PMID: 20200070
    18. Results provide evidence that PP2Cs are directly implicated in the ABA-dependent activation of OST1 PMID: 19855047
    19. Results suggest that negative regulation of KAT1 activity, required for stomatal closure, probably occurs by phosphorylation of KAT1 Thr306 by the stress-activated endogenous SnRK2.6 protein kinase. PMID: 19785574
    20. involved in the stomatal response to reduced relative humidity PMID: 16682349
    21. The identification of key residues involved in regulation OST1 activity is discussed. PMID: 16766677
    22. the function of UBA2a is independent of OST1 phosphorylation. PMID: 16828085

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Protein kinase superfamily, Ser/Thr protein kinase family
  • 组织特异性：
    Expressed in seedlings, leaves, flowers, stems, and roots, but restricted to guard cells and vascular tissue.
  • 数据库链接：

    KEGG: ath:AT4G33950

    STRING: 3702.AT4G33950.1

    UniGene: At.2399

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