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  4. Recombinant Arabidopsis thaliana Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic (RCA)

Recombinant Arabidopsis thaliana Ribulose bisphosphate carboxylase/oxygenase activase, chloroplastic (RCA)

  • 中文名称：
    拟南芥RCA重组蛋白
  • 货号：
    CSB-YP320802DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥RCA重组蛋白
  • 货号：
    CSB-EP320802DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥RCA重组蛋白
  • 货号：
    CSB-EP320802DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥RCA重组蛋白
  • 货号：
    CSB-BP320802DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥RCA重组蛋白
  • 货号：
    CSB-MP320802DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RCA
  • Uniprot No.：
    P10896
  • 别名：
    RCA; At2g39730; T5I7.3Ribulose bisphosphate carboxylase/oxygenase activase; chloroplastic; RA; RuBisCO activase
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Full Length of Mature Protein
  • 表达区域：
    59-474
  • 氨基酸序列
    AV KEDKQTDGDR WRGLAYDTSD DQQDITRGKG MVDSVFQAPM GTGTHHAVLS SYEYVSQGLR QYNLDNMMDG FYIAPAFMDK LVVHITKNFL TLPNIKVPLI LGIWGGKGQG KSFQCELVMA KMGINPIMMS AGELESGNAG EPAKLIRQRY REAADLIKKG KMCCLFINDL DAGAGRMGGT TQYTVNNQMV NATLMNIADN PTNVQLPGMY NKEENARVPI ICTGNDFSTL YAPLIRDGRM EKFYWAPTRE DRIGVCKGIF RTDKIKDEDI VTLVDQFPGQ SIDFFGALRA RVYDDEVRKF VESLGVEKIG KRLVNSREGP PVFEQPEMTY EKLMEYGNML VMEQENVKRV QLAETYLSQA ALGDANADAI GRGTFYGKGA QQVNLPVPEG CTDPVAENFD PTARSDDGTC VYNF
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Activation of RuBisCO (ribulose-1,5-bisphosphate carboxylase/oxygenase; EC 4.1.1.39) involves the ATP-dependent carboxylation of the epsilon-amino group of lysine leading to a carbamate structure.
  • 基因功能参考文献：
    1. Thecrystal structure of Rubisco activase from Arabidopsis thaliana is presented at 2.9 A resolution. PMID: 25849391
    2. Phosphorylation thus appears to be a potential mechanism associated with RCA dark inactivation, when Rubisco-catalysed carboxylation is arrested. PMID: 24119201
    3. manipulation of Rca regulation might provide a strategy for enhancing photosynthetic performance in certain variable light environments. PMID: 23417088
    4. These results confirmed the exceptional thermal lability of activase at physiological ratios of activase to Rubisco. PMID: 21728079
    5. Data suggested that the COI1-dependent JA repression of RCA played an important role in JA-induced leaf senescence. PMID: 21445012
    6. The COI1-dependent JA repression of RCA correlated with JA-induced leaf senescence. PMID: 21173027
    7. Differences in the thermal stability between wild type and mutant Rubisco activase were observed for the recombinant proteins and when the proteins were expressed in transgenic plants. PMID: 20450882
    8. Activase levels have a role in the thermotolerance of photosynthesis in Arabidopsis. PMID: 16997900
    9. Results provide clear evidence that RCA is a major limiting factor in plant photosynthesis under moderately elevated temperatures and a potential target for genetic manipulation to improve crop plants productivity under heat stress conditions. PMID: 17933901
    10. Transgenic lines expressing thermostable, chimeric activase showed higher rates of photosynthesis than the wild type after a short exposure to higher temperatures. PMID: 19507049

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  • 亚细胞定位：
    Plastid, chloroplast stroma. Plastid, chloroplast, plastoglobule.
  • 蛋白家族：
    RuBisCO activase family
  • 数据库链接：

    KEGG: ath:AT2G39730

    STRING: 3702.AT2G39730.1

    UniGene: At.25263

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