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  4. Recombinant Arabidopsis thaliana Retinoblastoma-related protein 1 (RBR1), partial

Recombinant Arabidopsis thaliana Retinoblastoma-related protein 1 (RBR1), partial

  • 中文名称：
    拟南芥RBR1重组蛋白
  • 货号：
    CSB-YP881749DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥RBR1重组蛋白
  • 货号：
    CSB-EP881749DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥RBR1重组蛋白
  • 货号：
    CSB-EP881749DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥RBR1重组蛋白
  • 货号：
    CSB-BP881749DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥RBR1重组蛋白
  • 货号：
    CSB-MP881749DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RBR1
  • Uniprot No.：
    Q9LKZ3
  • 别名：
    RBR1; RBR; At3g12280; F28J15.11; Retinoblastoma-related protein 1; AtRBR; AtRBR1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Key regulator of entry into cell division. Acts as a transcription repressor of E2F target genes, whose activity is required for progress from the G1 to the S phase of the cell cycle. Hyperphosphorylation by CDKA-1 prevents the binding to E2F transcription factors, allowing G1 to S phase transition to operate. Forms a stable complex with E2FA that functions in maintaining cell proliferation through repression of cell differentiation. Plays a central role in the mechanism controlling meristem cell differentiation, cell fate establishment and cell fate maintenance during organogenesis and gametogenesis. Required during lateral organ production. Also involved in controlling asymmetric divisions of stem cells in different stem cell niches. Acts as negative regulator of cell proliferation during leaf and gametophytes development. At later stages of development, restricts the progression through additional endocycles. In the leaf, plays a role in the control of the mesophyll differentiation. Another role is its implication in the regulation of imprinted genes. Acts together with MSI1 to repress the expression of MET1 during gametogenesis. This in turn activates expression of the imprinted genes FIS2 and FWA. Regulates many genes of the polycomb repressive complex 2 (PRC2). Plays an important role in meiosis affecting different aspects of this complex process. Functions as a positive regulator of the developmental switch from embryonic heterotrophic growth to autotrophic growth. Interaction with mastrevirus RepA or nanovirus Clink protein disrupts the RBR/E2F interaction and releases the transcription of replicative enzymes needed by the virus by increasing the E2F DNA-binding activity.
  • 基因功能参考文献：
    1. XND1 harbors an apparently angiosperm-specific combination of interaction motifs potentially linking the general differentiation regulator RBR with a xylem-specific pathway for inhibition of differentiation. PMID: 28742236
    2. One function of RBR1 is the direct repression of the stem cell factor WUSCHEL (WUS), which ectopically accumulates in meiocytes of triple krp and rbr1 mutants. PMID: 28450583
    3. The authors find that RBR1 is also required for RAD51 localization to DNA lesions. PMID: 28320735
    4. Together with E2FA, RBR is directly involved in the transcriptional DNA damage response as well as in the cell death pathway that is independent of SOG1, the plant functional analogue of p53. PMID: 28320736
    5. SIM and SMR1 are involved in hyperphosphorylation of the cell-cycle regulator RBR1 and overexpression of E2F target genes. PMID: 25455564
    6. Irreversible cell fate commitment in the Arabidopsis stomatal lineage requires a FAMA and RBR interaction. PMID: 25303364
    7. RBR protein stimulates cell differentiation in the Arabidopsis root meristem by interacting with cytokinin signaling. PMID: 24285791
    8. RBR is important in early leaf development and the extent to which physiology adapts to modified cellular architecture resulting from altered cell-cycle gene expression. PMID: 24118480
    9. RBR interacts with the stem cell transcription factor SCARECROW (SCR) through an LxCxE motif. PMID: 24302889
    10. S6K1 interacts with RBR via its N-terminal RBR binding motif, promotes its nuclear localization and consequent RBR-dependent repression of cell cycle genes through E2FB. PMID: 23531690
    11. RBR1 regulates not only cell cycle genes, but also, independent of the cell cycle transcription factor E2F, genes required for formative divisions and cell fate acquisition, thus directly linking cell proliferation with differentiation. PMID: 23104828
    12. Study tissue-generating asymmetric divisions in a stem cell daughter within the Arabidopsis root finds spatial restriction of these divisions requires physical binding of the stem cell regulator SCARECROW (SCR) by the RETINOBLASTOMA-RELATED (RBR) protein. PMID: 22921914
    13. AtRBR1 undergo proteasome-dependent degradation during sucrose starvation in Arabidopsis PMID: 22019639
    14. Data show that RBR has unique cell-autonomous functions in different cell types within the root stem cell niche. PMID: 21742994
    15. RBR is a positive regulator of the developmental switch from embryonic heterotrophic growth to autotrophic growth. PMID: 21693514
    16. Retinoblastoma protein is essential for early meiotic events in Arabidopsis. PMID: 21217641
    17. Conditional downregulation and loss of RBR function uncouples division and differentiation of meristemoid cells in leaves and disrupts the appropriate division and maintenance of meristem stem cells. PMID: 20525851
    18. RBR restricts cell division early during leaf development when cell proliferation predominates, while it regulates endocycle occurrence at later stages. PMID: 16361519
    19. This research shows that if there is a local reduction of the Retinoblastoma-Related Gene (RBR) in the roots of Arabidopsis roots this increases the amount of stem cells without affecting the cell cycle. PMID: 16377572
    20. EBP1 is a conserved, dose-dependent regulator of cell growth that is connected to meristematic competence and cell proliferation via regulation of RBR1 level. PMID: 17024182
    21. Results show that confirmed that downregulation of AtRBR1 leads to a prolonged G2 phase and delayed activation of G2/M marker genes. PMID: 18064404
    22. We have thus identified a new mechanism required for imprinting establishment, outlining a new role for the Retinoblastoma pathway, which may be conserved in mammals. PMID: 18700816
    23. RBR1 is required for cell differentiation of male and female gametophytes. PMID: 18976913

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  • 亚细胞定位：
    Nucleus.
  • 蛋白家族：
    Retinoblastoma protein (RB) family
  • 组织特异性：
    Expressed in actively dividing cells. Detected in the shoot apical meristem, in young leaf primordia and in both sporophytic tissue and the megagametophyte.
  • 数据库链接：

    KEGG: ath:AT3G12280

    STRING: 3702.AT3G12280.1

    UniGene: At.14958

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