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  4. Recombinant Arabidopsis thaliana Regulator of nonsense transcripts 1 homolog (UPF1), partial

Recombinant Arabidopsis thaliana Regulator of nonsense transcripts 1 homolog (UPF1), partial

  • 中文名称：
    拟南芥UPF1重组蛋白
  • 货号：
    CSB-YP875473DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥UPF1重组蛋白
  • 货号：
    CSB-EP875473DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥UPF1重组蛋白
  • 货号：
    CSB-EP875473DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥UPF1重组蛋白
  • 货号：
    CSB-BP875473DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥UPF1重组蛋白
  • 货号：
    CSB-MP875473DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    UPF1
  • Uniprot No.：
    Q9FJR0
  • 别名：
    UPF1; LBA1; At5g47010; MQD22.15; Regulator of nonsense transcripts 1 homolog; EC 3.6.4.-; ATP-dependent helicase UPF1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons (premature termination codon PTC) by associating with the nuclear exon junction complex (EJC) and serving as link between the EJC core and NMD machinery. Eliminates the production of nonsense-containing RNAs (ncRNAs). Required for plant development and adaptation to environmental stresses, including plant defense and response to wounding.
  • 基因功能参考文献：
    1. The involvement of UPF1, UPF2, and UPF3 in development and in response to stresses, wounding and infection by Pseudomonas syringae pv. tomato strain DC3000, were examined. PMID: 22353561
    2. UPF1 and UPF3, which are key nonsense-mediated mRNA decay factors, may act as defense-related regulators associated with plant immunity. PMID: 22025558
    3. AtUPF1 is involved in the control of seed size and shape and is essential for seedling growth. [AtUPF1] PMID: 16540482
    4. lba1 is a missense mutation of UPF1 RNA helicase involved in nonsense-mediated mRNA decay PMID: 16740149
    5. Nonsense-mediated mRNA decay and RNA interference (RNAi) in plants requires UPF1. [UPF1] PMID: 16813578
    6. Data show that in mutants impaired in the nonsense-mediated decay (NMD) components UPF1, levels of the alternatively spliced AtGRP7 and AtGRP8 transcripts are strongly elevated, implicating UPF1 in the decay of these clock-regulated transcripts. PMID: 17924945

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  • 亚细胞定位：
    Cytoplasm. Cytoplasm, P-body. Note=Remobilize from the cytoplasm into processing bodies by SMG7.
  • 蛋白家族：
    DNA2/NAM7 helicase family
  • 数据库链接：

    KEGG: ath:AT5G47010

    STRING: 3702.AT5G47010.1

    UniGene: At.8584

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