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  4. Recombinant Arabidopsis thaliana RNA-dependent RNA polymerase 2 (RDR2), partial

Recombinant Arabidopsis thaliana RNA-dependent RNA polymerase 2 (RDR2), partial

  • 中文名称：
    拟南芥RDR2重组蛋白
  • 货号：
    CSB-YP529348DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥RDR2重组蛋白
  • 货号：
    CSB-EP529348DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥RDR2重组蛋白
  • 货号：
    CSB-EP529348DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥RDR2重组蛋白
  • 货号：
    CSB-BP529348DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥RDR2重组蛋白
  • 货号：
    CSB-MP529348DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    RDR2
  • Uniprot No.：
    O82504
  • 别名：
    RDR2; RDRP2; SMD1; At4g11130; F2P3.11; RNA-dependent RNA polymerase 2; AtRDRP2; EC 2.7.7.48; Protein SILENCING MOVEMENT DEFICIENT 1; RNA-directed RNA polymerase 2
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    RNA-dependent direct polymerase involved in the production of small interfering RNAs (siRNAs). Required for the biogenesis of endogenous siRNAs of 24 nucleotide which derive from heterochromatin and DNA repeats such as transposons or endogenous gene tandem repeats, such as repeats present in FWA gene. Involved in transcriptional gene silencing (TGS). Component of the RNA-directed DNA methylation (RdDM) silencing pathway that utilizes siRNAs to guide DNA methyltransferases to asymmetric cytosines. Involved in control of flowering time through RdDM of FWA locus. Required for reception of long-distance mRNA silencing in the shoot. Required for the formation of telomeric siRNAs and the RNA-dependent DNA methylation of asymmetric cytosines in telomeric (5'-CCCTAAA-3') repeats.
  • 基因功能参考文献：
    1. JMJ24 binds to RDR2 and is required for the basal level transcription of silenced loci in Arabidopsis. PMID: 26119694
    2. These findings begin to reveal features of Pol IV/RDR2-mediated transcription at the heart of genome stability in plants. PMID: 25414514
    3. Results suggest that the physical and mechanistic coupling of Pol IV and RDR2 results in the channeled synthesis of double-stranded precursors for siRNA biogenesis. PMID: 23142082
    4. The data suggest a mechanism whereby IBM1 regulates a series of protein-coding genes including RDR2 through the RdDM-dependent control of siRNA biogenesis. PMID: 22772985
    5. RDR2 plays a significant role in regulating the expression of not only transposons, but also of genes. PMID: 19936292
    6. RDR2 is required for reception of long-distance mRNA silencing. PMID: 17785412

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  • 亚细胞定位：
    Nucleus, nucleoplasm. Nucleus, nucleolus.
  • 蛋白家族：
    RdRP family
  • 数据库链接：

    KEGG: ath:AT4G11130

    STRING: 3702.AT4G11130.1

    UniGene: At.43417

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