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  4. Recombinant Arabidopsis thaliana Pyrophosphate-energized vacuolar membrane proton pump 1 (AVP1), partial

Recombinant Arabidopsis thaliana Pyrophosphate-energized vacuolar membrane proton pump 1 (AVP1), partial

  • 中文名称：
    Recombinant Arabidopsis thaliana Pyrophosphate-energized vacuolar membrane proton pump 1(AVP1) ,partial
  • 货号：
    CSB-YP330080DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Pyrophosphate-energized vacuolar membrane proton pump 1(AVP1) ,partial
  • 货号：
    CSB-EP330080DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Pyrophosphate-energized vacuolar membrane proton pump 1(AVP1) ,partial
  • 货号：
    CSB-EP330080DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Pyrophosphate-energized vacuolar membrane proton pump 1(AVP1) ,partial
  • 货号：
    CSB-BP330080DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Pyrophosphate-energized vacuolar membrane proton pump 1(AVP1) ,partial
  • 货号：
    CSB-MP330080DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    AVP1
  • Uniprot No.：
    P31414
  • 别名：
    AVP1; AVP; AVP-3; AVP3; At1g15690; F7H2.3; Pyrophosphate-energized vacuolar membrane proton pump 1; EC 7.1.3.1; Pyrophosphate-energized inorganic pyrophosphatase 1; H(+)-PPase 1; Vacuolar proton pyrophosphatase 1; Vacuolar proton pyrophosphatase 3
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Contributes to the transtonoplast (from cytosol to vacuole lumen) H(+)-electrochemical potential difference. It establishes a proton gradient of similar and often greater magnitude than the H(+)-ATPase on the same membrane. In addition, facilitates auxin transport by modulating apoplastic pH and regulates auxin-mediated developmental processes. Confers tolerance to NaCl and to drought by increasing ion retention.
  • 基因功能参考文献：
    1. Transgenic Arabidopsis plants expressing both AVP1 and PP2A-C5 at relatively high levels were identified and analyzed. These plants displayed enhanced tolerance to NaCl compared to either AVP1 or PP2A-C5 overexpressing plants. PMID: 30080613
    2. AVP1 role in phloem loading and transport PMID: 26530315
    3. AVP1 localized at the plasma membrane of the sieve element-companion cell complexes functions as a synthase, and that this activity is critical for the maintenance of pyrophosphate homeostasis required for phloem function. PMID: 25681328
    4. AVP1DOX plants produced 25% more marketable ripened fruit per plant under P-deficient conditions compared with the controls. Further, under low phosphate conditions, they displayed increased phosphate transport from leaf (source) to fruit (sink). PMID: 24723407
    5. The expression of AVP1 increases the shoot biomass of barley in saline and nonsaline conditions. It is shown that the expression of AVP1 in transgenic barley improves the grain yield per plant of this cereal crop when grown in a high-salinity field. PMID: 24261956
    6. Results show that transgenic plants expressing higher levels of AVP1 transcripts in sugarcane are able to withstand salt and drought stress regimes. PMID: 24002911
    7. The characterization of romaine lettuce (Lactuca sativa 'Conquistador') plants engineered to overexpress an intragenic gain-of-function allele of the type I proton translocating pyrophosphatase (H(+)-PPase) of Arabidopsis, is reported. PMID: 23307651
    8. Hydrolysis of cytosolic PPi, rather than vacuolar acidification, is the major function of AVP1/FUGU5 in planta. Plant cells optimize their metabolic function by eliminating PPi in the cytosol for efficient postembryonic heterotrophic growth. PMID: 21862707
    9. Heightened expression of AVP1 enhances pyrophosphate-dependent proton pump activity, salt tolerance, ion vacuolar sequestration, K+ uptake capacity, root hair development, osmotic responses, and PMATPase hydrolytic and proton pumping activities. PMID: 22195582
    10. reported that the H+-pyrophosphatase, AVP1, controls auxin transport and consequently auxin-dependent development [AVP1] PMID: 16210544

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  • 亚细胞定位：
    Vacuole membrane; Multi-pass membrane protein. Endosome membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Note=Mostly vacuolar, tonoplast. Also present in endosomes and plasma membrane.
  • 蛋白家族：
    H(+)-translocating pyrophosphatase (TC 3.A.10) family, K(+)-stimulated subfamily
  • 组织特异性：
    Ubiquitous (at protein level). Mostly expressed in vascular tissues, meristems and root pericycle.
  • 数据库链接：

    KEGG: ath:AT1G15690

    STRING: 3702.AT1G15690.1

    UniGene: At.21942

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