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  4. Recombinant Arabidopsis thaliana Protein argonaute 7 (AGO7), partial

Recombinant Arabidopsis thaliana Protein argonaute 7 (AGO7), partial

  • 中文名称：
    Recombinant Arabidopsis thaliana Protein argonaute 7(AGO7) ,partial
  • 货号：
    CSB-YP866383DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Protein argonaute 7(AGO7) ,partial
  • 货号：
    CSB-EP866383DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Protein argonaute 7(AGO7) ,partial
  • 货号：
    CSB-EP866383DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Protein argonaute 7(AGO7) ,partial
  • 货号：
    CSB-BP866383DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Protein argonaute 7(AGO7) ,partial
  • 货号：
    CSB-MP866383DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    AGO7
  • Uniprot No.：
    Q9C793
  • 别名：
    AGO7; ZIP; At1g69440; F10D13.11Protein argonaute 7; Protein ZIPPY
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in RNA-mediated post-transcriptional gene silencing (PTGS). Main component of the RNA-induced silencing complex (RISC) that binds to a short guide RNA such as a microRNA (miRNA) or small interfering RNA (siRNA). RISC uses the mature miRNA or siRNA as a guide for slicer-directed cleavage of homologous mRNAs to repress gene expression. Required for the processing of 21 nucleotide trans-acting siRNAs (ta-siRNAs) derived from TAS3a transcripts. Associates preferentially with the microRNA (miRNA) miR390 which guides the cleavage of TAS3 precursor RNA. Seems to act as miR390 specific slicer. Associates mainly with small RNAs of 21 nucleotide in length and with a 5' terminal adenosine. Acts in the RDR6/SGS3/DCL4/AGO7 trans-acting siRNA pathway involved in leaf developmental timing. Does not seems to act on leaf polarity. Required for the production of the 30-40nt bacterial-induced long siRNAs (lsiRNA). Involved in antiviral RNA silencing by contributing to efficient viral RNAs clearance. Targets less structured viral RNAs than AGO1 which is capable of targeting RNAs with more compact structures.
  • 基因功能参考文献：
    1. Arabidopsis ARGONAUTE7 selects microRNA390 through multiple checkpoints during RISC assembly. PMID: 23732541
    2. It was shown that AGO7 is needed in the cytoplasm and membranous siRNA bodies for the processing of the TAS3 precursor, showing a hitherto unknown role for membrane-associated ribonucleoparticles in trans-acting-siRNA biogenesis and AGO action in plants. PMID: 22327216
    3. Study shows that miR390-ARGONAUTE7 complexes function in distinct cleavage or noncleavage modes at two target sites in TAS3a transcripts. PMID: 18342362
  • 蛋白家族：
    Argonaute family, Ago subfamily
  • 组织特异性：
    Expressed in leaves and floral buds, and at low levels in roots.
  • 数据库链接：

    KEGG: ath:AT1G69440

    STRING: 3702.AT1G69440.1

    UniGene: At.35421

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