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  4. Recombinant Arabidopsis thaliana Protein argonaute 2 (AGO2), partial

Recombinant Arabidopsis thaliana Protein argonaute 2 (AGO2), partial

  • 中文名称：
    Recombinant Arabidopsis thaliana Protein argonaute 2(AGO2) ,partial
  • 货号：
    CSB-YP871064DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Protein argonaute 2(AGO2) ,partial
  • 货号：
    CSB-EP871064DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Protein argonaute 2(AGO2) ,partial
  • 货号：
    CSB-EP871064DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Protein argonaute 2(AGO2) ,partial
  • 货号：
    CSB-BP871064DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Protein argonaute 2(AGO2) ,partial
  • 货号：
    CSB-MP871064DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    AGO2
  • Uniprot No.：
    Q9SHF3
  • 别名：
    AGO2; At1g31280; T19E23.7Protein argonaute 2
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in RNA-mediated post-transcriptional gene silencing (PTGS). Main component of the RNA-induced silencing complex (RISC) that binds to a short guide RNA such as microRNA (miRNA) or small interfering RNA (siRNA). RISC uses the mature miRNA or siRNA as a guide for slicer-directed cleavage of homologous mRNAs to repress gene expression. Associates mainly with siRNAs of 21 nucleotide in length and preferentially recruits small RNAs with a 5' terminal adenosine. Probably involved in antiviral RNA silencing. Associates with siRNA derived from cucumber mosaic virus (CMV). Targeted by turnip yellows virus (TuYV) protein P0 (via F-box-like domain) for probable proteasome degradation and thereby inactivating AGO2 function in RNA silencing. Required to direct NERD-dependent DNA methylation and silencing.
  • 基因功能参考文献：
    1. Data show that Argonaute family protein AGO2 and AGO3 were important for abscisic acid (ABA)-mediated resistance. PMID: 28270624
    2. Base pairing at the 15th nucleotide of a miRNA duplex is important for miRNA sorting in both Arabidopsis AGO1 and AGO2. AGO2 favours miRNA duplexes with no middle mismatches. PMID: 25406978
    3. Complementation analyses in ago mutant plants revealed that the catalytic residues of AGO1, AGO2, and AGO7 are required to restore the defects of Arabidopsis ago1-25, ago2-1, and zip-1 (AGO7-defective) mutants, respectively. PMID: 23023169
    4. AGO protein has evolved to specialize in antiviral defenses. PMID: 21576511
    5. AGO1 and AGO2 are involved in defense against mutant of Cucumber mosaic virus, which act downstream the biogenesis of viral secondary siRNAs in a nonredundant and cooperative manner. PMID: 21467580
    6. AGO2 may regulate and coordinate the expression of a group of genes involved in various pathways of plant immunity by binding to a group of functional sRNAs. [AGO2] [Argonaute 2] PMID: 21549312
    7. second layer is activated when the first layer is suppressed because AGO2 is repressed by AGO1 via miR403 PMID: 21305057

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  • 蛋白家族：
    Argonaute family, Ago subfamily
  • 数据库链接：

    KEGG: ath:AT1G31280

    STRING: 3702.AT1G31280.1

    UniGene: At.40380

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