Your Good Partner in Biology Research

中文简体

CSF2｜ CTLA4｜ DLL3｜ EGFR｜ IL4｜ IL4R｜ LAG3｜ MSTN｜ NECTIN4｜ NT5E｜ SEZ6｜ TM4SF1｜ TNFSF13｜ TPO

  1. 首页
  2. 蛋白
  3. 重组蛋白
  4. Recombinant Arabidopsis thaliana Protein argonaute 1 (AGO1), partial

Recombinant Arabidopsis thaliana Protein argonaute 1 (AGO1), partial

  • 中文名称：
    Recombinant Arabidopsis thaliana Protein argonaute 1(AGO1) ,partial
  • 货号：
    CSB-YP520796DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Protein argonaute 1(AGO1) ,partial
  • 货号：
    CSB-EP520796DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Protein argonaute 1(AGO1) ,partial
  • 货号：
    CSB-EP520796DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Protein argonaute 1(AGO1) ,partial
  • 货号：
    CSB-BP520796DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    Recombinant Arabidopsis thaliana Protein argonaute 1(AGO1) ,partial
  • 货号：
    CSB-MP520796DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    AGO1
  • Uniprot No.：
    O04379
  • 别名：
    AGO1; At1g48410; F11A17.3; T1N15.2Protein argonaute 1
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Involved in RNA-mediated post-transcriptional gene silencing (PTGS). Main component of the RNA-induced silencing complex (RISC) that binds to a short guide RNA such as microRNA (miRNA) or small interfering RNA (siRNA). RISC uses the mature miRNA or siRNA as a guide for slicer-directed cleavage of homologous mRNAs to repress gene expression. Requires DRB1 for directional loading of the small RNA duplex (guide stand and passenger strand) onto RISC for passenger strand degradation. Unlike animal RISC that associates in high molecular weight complex, Arabidopsis RISC is probably composed only of the AGO1 protein and associated RNA without any other proteins. Associates mainly with miRNAs of 21 nucleotide in length and preferentially recruits small RNAs with a 5' terminal uridine. Associates with 22 nucleotide miRNAs to trigger RDR6-dependent secondary siRNAs biogenesis. This pathway amplifies silencing by using the target RNA as substrate to generate secondary siRNAs. Binds to miR168 which targets its own mRNA for repression, establishing a homeostatic regulatory loop. Involved in antiviral RNA silencing by contributing to viral RNA clearance. Is capable of targeting viral RNAs with more compact structures than AGO7 which favors less structured RNA targets. May not associate with 24 nucleotide siRNAs involved in chromatin silencing. Essential for multiple processes in development. Essential for proper development of leaves and floral organs, and formation of axillary meristems. Like AGO10, required for stem cell function and organ polarity.
  • 基因功能参考文献：
    1. AGO1 role in the repair of UV-induced DNA lesions PMID: 28325872
    2. Here is described the identification of AGO1 association with polysomes through polysome fractionation on sucrose gradient, preparation of proteins by filtration and concentration, and immunoblotting. PMID: 28608339
    3. Sweet potato mild mottle virus P1 interacts with AGO1 and AGO2, but solely interferes with AGO1 function. PMID: 28499009
    4. Results suggests that ARGONAUTE1 (AGO1) interacts with chromatin at microRNAs MIR161 and MIR173 loci and causes the disassembly of the transcriptional complex, releasing short and unpolyadenylated transcripts. PMID: 27385819
    5. that AGO1 may have functions in gene regulation independent of small RNAs. PMID: 27208258
    6. AGO1 is a major, largely HSP90-independent, factor in providing environmental robustness to plants. PMID: 26872948
    7. miR168 is a key miRNA because it regulates the expression of the slicer protein ARGONAUTE1 (AGO1), which catalyzes mRNA cleavage. Interestingly, plant miR168s are highly evolutionarily conserved; however, it is unclear whether MIR168 promoter elements and expression patterns are also conserved. PMID: 27323093
    8. a total of 5,123 and 1,399 AGO1-enriched small RNAs, excluding miRNAs, were identified in Arabidopsis thaliana and rice (Oryza sativa), respectively. PMID: 26206233
    9. Mungbean yellow mosaic Indian virus AC2 interacts with AGO1 and inhibits its transcript slicing activity in vitro. PMID: 26433748
    10. Base pairing at the 15th nucleotide of a miRNA duplex is important for miRNA sorting in both Arabidopsis AGO1 and AGO2. AGO1 tolerates, or prefers, duplexes with central mismatches. PMID: 25406978
    11. In the presence of the wild-type allele of AGO1, most of the mas mutations cause a mild or no mutant phenotype on their own, indicating that the ago1-52 mutant may provide a sensitized background for examining the interactions of AGO1. PMID: 24985352
    12. AGO1 is required to restrict AGL16-mediated stomatal spacing divisions, an miRNA pathway in addition to ligand-receptor signaling modules. PMID: 24386951
    13. methylation is required to protect miRNAs from AGO1-associated HESO1 activity that normally uridylates 5' fragments. PMID: 24733911
    14. a selective process such as ubiquitylation can lead to the degradation of a key regulatory protein such as AGO1 by a degradation process generally believed to be unspecific PMID: 23019378
    15. The contrasted effects of viral RNA silencing suppressors on siRNA versus miRNA loading into Argonaute1 imply the existence of two distinct pools of cellular Argonaute1 that are specifically loaded by each class of RNAs. PMID: 22531783
    16. EMA1 modulates miRNA activity by influencing the loading of miRNAs into AGO1 complexes. PMID: 21984696
    17. Transcriptional elevation of MIR168a is required for maintaining a stable AGO1 transcript level during the stress response. PMID: 22247272
    18. P0 protein is a silencing suppressor which inhibits local and systemic RNA silencing through AGO1 degradation. PMID: 22361475
    19. provide evidence that AGO1 is a peripheral membrane protein. Moreover, specific hypomorphic mutant alleles of AGO1 display compromised membrane association and AGO1-membrane interaction is reduced upon knockdown of HMG1/MAD3 PMID: 22247288
    20. DNA-dependent RNA polymerase V-dependent smRNAs were 21 nt and bound to AGO1, suggesting the potential involvement of AGO1 in Pol V-related pathways. PMID: 21457371
    21. The P38 GW residues bind directly and specifically to Arabidopsis AGO1, which, in addition to its role in endogenous microRNA-mediated silencing, is identified as a major effector of TCV-derived siRNAs PMID: 20439431
    22. Stduies indicate that RNA silencing can be transmitted between cells through AGO1. PMID: 19822452
    23. Thus, we suggest that ARF17 negatively regulates adventitious root formation in ago1 mutants by repressing GH3 genes and therefore perturbing auxin homeostasis in a light-dependent manner PMID: 15829601
    24. AGO1 has a role in meristem formation and identity PMID: 15882589
    25. Slicer activity fractionates as a complex of approximately 150 kDa that likely constitutes the ARGONAUTE1 protein and associated RNA without any other proteins [ARGONAUTE1] [AGO1] PMID: 16081530
    26. AGO1 is required for repressing several class I KNOTTED1-like homeobox (KNOX) genes in leaves, and for specifying both adaxial and abaxial identities of the leaf and petal. PMID: 16786292
    27. Genetic supprsesion blocks AGO1 cleavage activity to inhibit miRNA pathways, attenuate RNA silencing, and counter host defense PMID: 17158744
    28. miR173 functions in association with AGO1 during TAS1 and TAS2 tasiRNA formation, and data indicates that the miR173-AGO1 complex possesses unique functionality that many other miRNA-AGO1 complexes lack. PMID: 19066226
    29. Loss of function of AGO1 perturbed the radial symmetry of Arabidopsis root ground tissue. PMID: 19188262
    30. Data show that siRNA-mediated AGO1 silencing depends on correct miRNA targeting, pointing to coordinated regulatory actions of the miRNA and siRNA pathways during the maintenance of AGO1 homeostasis. PMID: 19343050
    31. results suggest that 22-nt miR168 ensures basal cleavage of AGO1 mRNA whereas 21-nt miR168 permits an effective response to endogenous or environmental fluctuations owing to its preferential stabilization by AGO1 PMID: 19649244

    显示更多

    收起更多

  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Argonaute family, Ago subfamily
  • 组织特异性：
    Widely expressed at low levels.
  • 数据库链接：

    KEGG: ath:AT1G48410

    STRING: 3702.AT1G48410.2

    UniGene: At.21124

Most popular with customers

产品中心
特别关注


X
九游平台-九游(中国) | 米兰体育-米兰体育（中国） | 开云手机在线登陆入口 | 江南官方体育网页版 | 开云网页版 | 开云官方在线入口 | 开云电子体育 | 开云官方链接 | 江南网页版页面登录 |