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  4. Recombinant Arabidopsis thaliana Protein arginine N-methyltransferase 1.5 (PMRT15), partial

Recombinant Arabidopsis thaliana Protein arginine N-methyltransferase 1.5 (PMRT15), partial

  • 中文名称：
    拟南芥PMRT15重组蛋白
  • 货号：
    CSB-YP812747DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥PMRT15重组蛋白
  • 货号：
    CSB-EP812747DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥PMRT15重组蛋白
  • 货号：
    CSB-EP812747DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥PMRT15重组蛋白
  • 货号：
    CSB-BP812747DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥PMRT15重组蛋白
  • 货号：
    CSB-MP812747DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    PMRT15
  • Uniprot No.：
    Q8GWT4
  • 别名：
    PMRT15; PMRT5; SKB1; At4g31120; F6E21.40Protein arginine N-methyltransferase 1.5; AtPMRT15; AtPMRT5; EC 2.1.1.319; Shk1 kinase-binding protein 1 homolog
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

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PMRT15 antibodies

靶点详情

  • 功能：
    Methylates arginine residues of myelin basic protein (MBP) in vitro. Methylates symmetrically histone H4 of the FLC chromatin to form H4R3me2s, which in turn suppresses FLC expression to induce flowering. Regulates alternative splicing by methylating spliceosomal proteins. Involved in the post-transcriptional regulation of the circadian clock.
  • 基因功能参考文献：
    1. AtPRMT5 Regulates Shoot Regeneration through Mediating Histone H4R3 Dimethylation on KRPs and Pre-mRNA Splicing of RKP in Arabidopsis PMID: 27780782
    2. a new mechanism of plant root stem cell maintenance under DNA damage conditions that requires SKB1 PMID: 27090604
    3. Plants transduce stress-triggered NO signal to protein methylation machinery through S-nitrosylation of PRMT5 in response to environmental alterations. PMID: 28757206
    4. SKB1 and SKB1-mediated H4R3sme2 are required for the maintenance of SAM in Arabidopsis seedlings. PMID: 24349476
    5. A mutation in CAU1 significantly increased the expression level of the calcium signaling gene CAS, and functional disruption of CAS abolished the enhanced drought tolerance and stomatal [Ca(2+)]o signaling in cau1. PMID: 23943859
    6. SKB1 methylates the U6 snRNP LSM4 in response to salt stress and ABA. Loss of SKB1 influences pre-mRNA splicing. PMID: 21258002
    7. period lengthening associated with mutational disruption of prmt5 indicates that Rsme2 is a decoration important for the Arabidopsis clock PMID: 21097700
    8. key role for PRMT5 in the regulation of alternative splicing PMID: 20962777
    9. AtPRMT5 deficiency causes splicing defects in hundreds of genes involved in multiple biological processes. The splicing defects are identified in transcripts of several RNA processing factors involved in regulating flowering time. PMID: 20956294
    10. SKB1-mediated H4R3sme2 is a novel histone mark required for repression of FLC expression and flowering time control. PMID: 17363895
    11. involved in promotion of vegetative growth and FlOWERING LOCUS C-dependent flowering time regulation PMID: 17573539

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  • 亚细胞定位：
    Cytoplasm.
  • 蛋白家族：
    Class I-like SAM-binding methyltransferase superfamily, Protein arginine N-methyltransferase family
  • 组织特异性：
    Highly expressed in flowers, roots and siliques, lower expression in stems and mature leaves (at protein level). Abundant in shoot apex, young leaves and leaf primordia, floral and inflorescence meristems, gynoecium, stamens, sepals and young siliques, bu
  • 数据库链接：

    KEGG: ath:AT4G31120

    STRING: 3702.AT4G31120.1

    UniGene: At.31771

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