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  4. Recombinant Arabidopsis thaliana Protein VERNALIZATION INSENSITIVE 3 (VIN3), partial

Recombinant Arabidopsis thaliana Protein VERNALIZATION INSENSITIVE 3 (VIN3), partial

  • 中文名称：
    拟南芥VIN3重组蛋白
  • 货号：
    CSB-YP880749DOA
  • 规格：
  • 来源：
    Yeast
  • 其他：
  • 中文名称：
    拟南芥VIN3重组蛋白
  • 货号：
    CSB-EP880749DOA
  • 规格：
  • 来源：
    E.coli
  • 其他：
  • 中文名称：
    拟南芥VIN3重组蛋白
  • 货号：
    CSB-EP880749DOA-B
  • 规格：
  • 来源：
    E.coli
  • 共轭：
    Avi-tag Biotinylated

    E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.

  • 其他：
  • 中文名称：
    拟南芥VIN3重组蛋白
  • 货号：
    CSB-BP880749DOA
  • 规格：
  • 来源：
    Baculovirus
  • 其他：
  • 中文名称：
    拟南芥VIN3重组蛋白
  • 货号：
    CSB-MP880749DOA
  • 规格：
  • 来源：
    Mammalian cell
  • 其他：

产品详情

  • 纯度：
    >85% (SDS-PAGE)
  • 基因名：
    VIN3
  • Uniprot No.：
    Q9FIE3
  • 别名：
    VIN3; At5g57380; MSF19.4Protein VERNALIZATION INSENSITIVE 3
  • 种属：
    Arabidopsis thaliana (Mouse-ear cress)
  • 蛋白长度：
    Partial
  • 蛋白标签：
    Tag type will be determined during the manufacturing process.
    The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
  • 产品提供形式：
    Lyophilized powder
    Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
  • 复溶：
    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
  • 储存条件：
    Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
  • 保质期：
    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
    Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
  • 货期：
    Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
    Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
  • 注意事项：
    Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
  • Datasheet ：
    Please contact us to get it.

产品评价

靶点详情

  • 功能：
    Plays a central role in vernalization by mediating the initial transcriptional repression of the homeotic gene FLC, a floral repressor, after a cold treatment. However, due to its transient expression, it cannot maintain repression of FLC, which is then maintained by Polycomb Group complexes containing VRN2 throughout development. Required to deacetylate histones on the FLC promoter. Together with VIL1, required during vernalization for the modifications of FLC and FLM chromatin that are associated with an epigenetically silenced state (e.g. chromatin modifications, histone deacetylation, and trimethylated H3 'Lys-4' H3K4me3 and 'Lys-27' H3K27me3) and with acquisition of competence to flower.
  • 基因功能参考文献：
    1. The binding specificity of the PHD-finger domain of VIN3 plays a role in mediating a proper vernalization response in Arabidopsis. PMID: 27999085
    2. VIN3 family of proteins nonredundantly functions to repress different subsets of the FLC gene family during the course of vernalization. PMID: 23417034
    3. The chain of events leading to the expression of VIN3, including changes at the chromatin level, are reported. PMID: 21051939
    4. VIN3 is under a constitutively repressed state, which is transiently relieved from repression only when sufficient cold is provided. PMID: 20671111
    5. During short-term cold exposure, histone H3 acetylation at the transcription start site rapidly increased, implying that it is required for VIN3 induction. PMID: 19825652
    6. VRN2 and VIN3 are part of a large protein complex that can include the PcG proteins FERTILIZATION INDEPENDENT ENDOSPERM, CURLY LEAF, and SWINGER, that is responsible for histone deacetylation at FLC and histone methylation at FLC in vernalized plants. PMID: 16983073
    7. VIN3 is induced in response to hypoxic conditions, but is not induced in response to other stresses that affect redox homeostasis. PMID: 19392705

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  • 亚细胞定位：
    Nucleus. Nucleus speckle. Note=Probably DNA-associated; interacts directly with the FLC regulatory regions.
  • 组织特异性：
    Expressed in shoot and root apices. Displays the same pattern of expression as FLC.
  • 数据库链接：

    KEGG: ath:AT5G57380

    STRING: 3702.AT5G57380.1

    UniGene: At.22442

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